Pharmacodynamic Research of Tongmai Oral Liquid on Preventing and CuringⅡPeriod Diabetic Nephropathy / 中国中医药信息杂志

Objective To investigate the effect of Tongmai oral liquid on the early diabetic nephropathy. Methods Diabetic nephropathy rats were induced by excision of the right kidney and a single intraperitoneal injection of streptozotocin (STZ). Forty male Wistar rats were divided randomly into 3 groups:Tongmai group, nephrotic model group, and normal group. At the end of the 8th week, the rats general states and some parameters such as kidney hypertrophy index, 24 h urine volume, blood glucose, 24 h urinary microalbumin (24 h UMA), renal function [serum creatinine (Cr), blood urea nitrogen (BUN)], serum lipid, and the change of nephritic pathologic lesion were measured. Result Compared with the model group, general states in Tongmai group were improved, there was a considerable increase on body weight (P

Preparation and Quality Control of Tongmai Oral Liquid / 中国药房

OBJECTIVE:To prepare tongmai oral liquid and study its quality control method.METHODS:The danshen root in tongmai oral liquid was identified by TLC.The content of ferulic acid in the oral liquid was determined by HPLC.RESULTS:Danshen root showed a good specificity in qualitation identification.The linear range of ferulic acid was5.12～71.68?g/ml(r=0.9998).The mean recovery was99.96%(RSD=1.3%).CONCLUSIONS:The preparation technology is practicable and quality control method is reliable.

Effect of Tongmai Oral Liquid on Renal Pathological Feature and Tumor Growth Factor ?_1 in Rats with Experimental Diabetic Nephropathy / 广州中医药大学学报

Objective To observe the effect of Tongmai Oral Liquid(TOL) on renal pathological feature and tumor growth factor ?1(TGF-?1) in rats with experimental diabetic nephropathy(DN).Methods Forty SD male rats were used for the experiment.Of which,8 were in the normal group,and the other rats were given one-dose intraperitoneal injection of streptozocin(STZ),and were fed with high-fat forage every other day.The normal group received one-dose intraperitoneal injection of aseptic citric acid-sodium citrate buffer and routine forage.After feeding for 4 weeks,the rat models of DN were randomized into the model group and TOL group,which were given normal saline and TOL(2.5 g?kg-1?d-1) respectively,and the two groups were still fed with high-fat forage.After treatment for 6 weeks,the rats were executed and the pathological features and ultrastructure of renal tissue were observed under light microscope and electron microscope respectively.Mean glomerular area(MGA),mean extracellular matrix area(MMA),and the thickness of glomerular basement membrane(GBM) as well as the ratio of MMA/MGA were measured.Meanwhile,the expression of tumor growth factor ?1(TGF-?1) was detected by immunohistochemical method.Results In TOL group,the pathological features such as broadened mesentery,thick basement membrane and vacuolar degeneration in the renal tubular epithelium were relieved,MMA/MGA ratio was decreased,GBM became thin,and the expression of TGF-?1 was strong mainly in renal tubular and interstitial region while weak in glomeruli,and its area density and integral optimal density were decreased as compared with the model group(P

Pharmacodynamic Study of Tongmai Oral Liquid for Chronic Nephritis Rat Model with Qi- deficiency and Blood-stasis Syndrome / 广州中医药大学学报

The mechanism of Tongmai Oral Liquid (TOL) for chronic nephritis rat model with Qi-deficiency and blood-stasis syndrome was studied.The model was established by adding Qi-deficiency and blood-stasis syndrome to the chronic nephritis rats. The experimental rats were randomly allocated to 5 groups: Group A (treated with high dosage of TOL), Group B (treated with low dosage of TOL), Group C (treated with Jinshuibao), Group D (model control group) and Group E (normal control group). After 4 weeks of treatment, general health state, biochemical indexes including T lymphocyte subgroup and blood rheology, and pathological damage of kidney tissue were much improved in Group A than Group B and Group C. It is indicated that TOL can improve the renal function and delay the occurrence of glomerular arteriosclerosis in rats by reinforcing Qi, activating blood flow, regulating immune function, lessening the hypercoagulative state and reducing renal damage.

Experimental Study on Tongmai Oral Liquid Prepared by Different Processes / 中成药

Objective: To study various preparation methods for Tongmai Oral Liquid. Methods: Tongmai Oral Liquid with ultrafiltration method, water alcohol method, talcum powder method, and method were prepared respectively; using impurity clearing efficency, clarity, total saponin content as parameters for quality control. Results: Tongmai Oral Liquid prepared by water alcohol method (alcohol concentration being 75%) was better than that by other methods in claerity, and stability and has highest total saponin. Conclusions: Quality of Tongmai Oral Liquid prepared by water alcohol method was available.