An Analysis of YU Hai-Bo's Experience in Treating Paediatric Cerebral Palsy by Using"Jianpi Yishen Triple-Needle Grouping Acupoints" / 广州中医药大学学报

This article introduces the clinical approach and acupuncture characteristics of the traditional Chinese medicine practitioner Professor YU Hai-Bo in treating paediatric cerebral palsy using the"Jianpi Yishen Triple-Needle Grouping Acupoints".Guided by the theory of growth and development of"viscera-meridian-brain"growth and development,Professor YU believed that"insufficiency of spleen and kidney"is the core pathogenesis of paediatric cerebral palsy,and the treatment concept of"treating from the spleen and kidney"was proposed.He inherited and innovated the triple-needle grouping acupoints therapy and establishing the system of"Jianpi Yishen Triple-Needle Grouping Acupoints".Before regular acupuncture,the abdomen and dorsum are pricked to freely regulate the middle energizer,and the upper limbs are selected as"Hegu(LI4),Waiguan(SJ5),Quchi[(LI11),three acupoints on the hand]+ Neiguan(PC6)";the lower limbs are selected as"Zusanli(ST36),Sanyinjiao(SP6),Taichong[(LR3),three acupoints on the foot];"Shenmai(BL62),Zhaohai(KI6),Yongquan(KI1)",spleen and kidney are regulated simultaneously,and the head acupoints include Sishencong(EX-HN1),intelligence tri-needling,cerebral tri-needling,temporal tri-needling,mind-calming needling and bilateral Fengchi(GB20).In order to regulate the spirit and benefit the intellect,the matching acupoints are modified according to the disease and the syndromes.At the same time,it is supplemented with music therapy and auricular point seed-pressing.Emphasis is placed on the simultaneous regulation of"child-parent-doctor"and"treating the person"rather than the"treating the disease".

YANG Yu-Feng's Experience in Treating Seborrheic Alopecia Using the Method of Strengthening the Spleen,Dispelling Dampness,Controlling Oil and Removing Fat / 广州中医药大学学报

Based on the theory of traditional Chinese medicine(TCM)and clinical manifestations of seborrheic alopecia,Professor YANG Yu-Feng believes that the fundamental pathogenesis of seborrheic alopecia is due to spleen deficiency with dampness accumulation,and dampness and fat sharing the same origin.The internal accumulation of dampness and turbidity results from the deficiency of spleen,and then the dampness and turbidity blended with heat attack the head and face.For dampness and fat share the same origin,the turbid fat forms,and then overflows and blocks the hair roots,which causes the qi and blood being difficult to go upward and nourish the hair roots,and then induces hair loss.Among them,spleen deficiency with dampness accumulation is the core pathogenesis of the disease,heat activating the movement of dampness is the key pathogenesis to the disease,and the disharmony of qi and blood is the pivot of the pathogenesis.For the treatment of seborrheic alopecia,Professor YANG Yu-Feng suggested that the hair root should be unblocked and the hair quality should be nourished by blood essence,and emphasized the importance of the method of invigorating the spleen and removing dampness,controlling oil and removing fat in dredging the hair root and relieving seborrheic alopecia.It is proposed that invigorating the spleen and removing dampness is the key,expelling wind and clearing heat is for assistance,and harmonizing qi and blood serves as the pivot.

On the Time Value of Yu Weihan’s Spiritual Strength / 中国医学伦理学

Yu Weihan, academician of the Chinese Academy of Engineering, devoted his whole life to the prevention and control of endemic diseases and basically eradicated the epidemic of Keshan disease. Academician Yu Weihan’s struggle experience has left us with valuable spiritual wealth. His spiritual strength is manifested in many aspects, such as the spirit of bearing hardships first, the spirit of innovative daring to be the first, the spirit of craftsmen striving for perfection, the patriotic spirit of caring for the people, and the dedication of being indifferent to fame and wealth. His great medical feelings of bravely shouldering the mission, caring for the people and taking root in the grass-roots have forcefully answered the three important questions: what kind of doctor to be, who to be and how to be a good doctor in the learning and working career of doctors in the new era. He set an model for doctors in later generations and highlighted the value of this spiritual strength for the time.

Therapeutic effect of Xiayuxue decoction on a mouse model of nonalcoholic fatty liver disease induced by high-fat diet and its mechanism / 临床肝胆病杂志

ObjectiveTo investigate the mechanism of action of Xiayuxue decoction in inhibiting nonalcoholic fatty liver disease （NAFLD） induced by high-fat diet in mice by regulating nucleotide binding oligomerization domain like receptor containing pyrin domain protein 6 （NLRP6）. MethodsA total of 15 male C57BL/6 mice were randomly divided into low-fat diet （LFD） group， high-fat diet （HFD） group， and Xiayuxue decoction-HFD group （XYXD group）， with 5 mice in each group. Liver function parameters （alanine aminotransferase ［ALT］ and aspartate aminotransferase ［AST］） and blood lipid metabolic indicators （triglycerides ［TG］ and total cholesterol ［TC］） were measured； HE staining and oil red O staining were performed for liver tissue to observe histomorpholoty and lipid droplet deposition； quantitative real-time PCR was used to measure the expression levels of inflammatory factors （tumor necrosis factor-α ［TNF-α］， interleukin-1β ［IL-1β］， interleukin-18 ［IL-18］， and NLRP6） in liver tissue； Western blot was used to measure the protein expression levels of NLRP6， nuclear factor-kappa B （NF-κB）， and NF-κB p65； immunohistochemistry was used to measure the expression of NLRP6 and CD68. Mouse Raw264.7 cells were treated with palmitic acid （PA）， lipopolysaccharide， and serum containing Xiayuxue decoction to observe inflammation. A one-way analysis of variance was used for comparison of continuous data between multiple groups， and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the LFD group， the HFD group had significant increases in the serum levels of ALT， AST， TC， and TG （all P<0.05）. Liver histopathological examination showed that the HFD group had marked hepatic steatosis and a signficant increase in NAS score （P<0.05）， and quantitative real-time PCR showed significant increases in the inflammatory factors such as IL1β and IL-18 and a significant reduction in the expression of NLRP6 （all P<0.05）. Immunohistochemistry showed that the expression of NLRP6 showed a similar trend as that of the macrophage marker CD68. Western blot showed that after the downregulation of NLRP6 expression， there was a significant increase in phosphorylated NF-κB p65 （P<0.05）. Compared with the HFD group， Xiayuxue decoction effectively improved liver inflammation， upregulated the expression of NLRP6， and downregulated phosphorylated NF-κB p65 in HFD mice （all P<0.05）. After Raw264.7 cells were treated with PA， NLRP6 was downregulated to promote the progression of inflammation （P<0.05）， and treatment with Xiayuxue decoction could upregulate NLRP6 and inhibit inflammation NF-κB （P<0.05）. ConclusionXiayuxue decoction can effectively improve hepatic steatosis and liver inflammation in a mouse model of NAFLD， possibly by regulating NLRP6/NF-κB to alleviate macrophage activation.

The effect of Yu's Qingre Huoxue prescription on colonic mucosa of ulcerative colitis model rats / 中国现代医生

@#Objective To investigate the effect of Yu's Qingre Huoxue prescription enema on colonic mucosa in rats with ulcerative colitis(UC).Methods A total of 30 Wistar rats were randomly divided into blank control group,model group,mesalazine group,Chinese medicine high-dose,medium-dose and low-dose groups(5 rats in each group).After successful modeling with trinitrobenzene sulfonic acid,the rats were given 14 days enema with distilled water,mesalazine suspension,and high,medium and low concentration Yu's Qingre Huoxue prescription.The general condition and weight changes of rats were recorded daily,and colon macroscopic damage index(CMDI)and mucosal injury score under microscope of rats in each group were compared.Results Compared with blank control group,the weight of rats in model group decreased significantly(P<0.05).The blank control group had normal water and food intake and activity,glossy fur and normal stool.The rats in model group and each administration group were found to have anal fouling,residual blood in stool,decreased appetite,messy fur,dim luster and other conditions,and the above conditions were gradually improved after enema treatment.Compared with blank control group,the CMDI and mucosal injury scores of model group were significantly increased(P<0.05).Compared with model group,CMDI and mucosal injury scores of mesalazine group and Chinese medicine high-dose,medium-dose and low-dose groups were significantly decreased(P<0.05).Conclusion Yu's Qingre Huoxue prescription can effectively improve the clinical symptoms of diarrhea,mucous,pus and blood stools in UC model rats,reduce the damage of colonic mucosa,promote mucosal repair,and have a good effect on the treatment of UC in rats.

Micro duelo: adaptação do jogo Yu-Gi-Oh para o ensino da Microbiologia / Micro duel: adaptation of the game Yu-Gi-Oh for teaching Microbiology / Micro duelo: adaptación del juego Yu-Gi-Oh para la enseñanza de Microbiología

Objetivo: Relatar a experiência de professores tutores e professores da Educação Básica, participantes do curso EAD Ensino de Ciências para a Promoção de Saúde na Escola Básica, na construção de um material didático-pedagógico para ser utilizado em sala de aula. Método: Para diversificar a rotina de aulas no ensino de Ciências e Biologia, direcionadas para alunos do ensino médio, os professores da Educação Básica que trabalharam os conteúdos do módulo de Saúde e Educação, desenvolveram um jogo de cartas que aborda a microbiologia, inspirado em Yu-Gi-Oh, uma série animada de mangá (histórias japonesas em quadrinhos), onde os jogadores usam cartas para duelar, em uma batalha simulada. Resultados: O material didático descreve os principais conceitos utilizados nojogo (duelo, pontos de vida, tipos de cartas etc.), as fases do duelo e os passos para iniciar o jogo. As cartas foram adaptadas para os assuntos abordados na microbiologia e continham nome do agente, tipo, descrição, atributo em cores, nível de ação em estrelas, número da carta e os pontos de ataque e de defesa. Conclusão: Espera-se estimular a interação e o aprendizado dos estudantes, através da aquisição de habilidades indispensáveis ao desenvolvimento intelectual e facilitação dos conceitos relacionados à microbiologia.

Objective: Report the experience of tutors and Basic Education teachers, participants in the EAD course Teaching Science for Health Promotion in Basic Schools, in the construction of didactic-pedagogical material to be used in the classroom. Method: To diversify the routine of teaching Science and Biology classes, aimed at high school students, the Basic Education teachers who worked on the contents of the Health and Education module, developed a card game that addresses microbiology, inspired by Yu-Gi-Oh, an animated manga series (Japanese comic books), where players use cards to duel in a simulated battle. Results: The teaching material describes the main concepts used in the game (duel, life points, types of cards, etc.), the phases of the duel and the steps to start the game. The cards were adapted to the subjects covered in microbiology and contained the agent's name, type, description, attribute in colors, action level in stars, card number and attack and defense points. Conclusion: It is expected to stimulate student interaction and learning, through the acquisition of skills essential for intellectual development and facilitation of concepts related to microbiology.

Objetivo: Reportar la experiencia de tutores y docentes de Educación Básica, participantes del curso EADEnseñanza de Ciencias para la Promoción de la Salud en Escuelas Básicas, en la construcción de material didáctico-pedagógico para ser utilizado en aula. Método: Para diversificar la rutina de enseñanza de las clases de Ciencias y Biología, dirigidas a estudiantes de secundaria, los docentes de Educación Básica que trabajaron los contenidos Salud y Educación desarrollaron un juego de cartas que aborda la microbiología, inspirado en Yu-Gi-Oh, una serie animada de manga (cómics japoneses), donde los jugadores usan cartas para batirse en duelo en una batalla simulada. Resultados: El material didáctico describe los principales conceptos utilizados en el juego (duelo, puntos de vida, tipos de cartas, etc.), las fases del duelo y los pasos para iniciar el juego. Las tarjetas estaban adaptadas a los temas tratados en microbiología y contenían el nombre del agente, tipo, descripción, atributo en colores, nivel de acción en estrellas, número de tarjeta y puntos de ataque y defensa. Conclusión: Se espera estimular la interacción y el aprendizaje de los estudiantes, a través de la adquisición de habilidades esenciales para el desarrollo intelectual y la facilitación de conceptos relacionados con la microbiología.

Clinical efficacy of entecavir combined with Biejiajian pills and its influence on TCM syndrome scores during the treatment of chronic hepatitis B with hepatic fibrosis and blood stasis syndrome / 中华肝脏病杂志

Objective: To investigate the clinical efficacy of entecavir combined with Biejiajian pills and its influence on TCM syndrome scores during the treatment of chronic hepatitis B with hepatic fibrosis and blood stasis syndrome by prospective, randomized and controlled study. Methods: Patients with chronic hepatitis B with hepatic fibrosis and blood stasis syndrome were selected as the research subjects and randomly divided into a treatment group and a control group. Entecavir plus Biejiajian pills or entecavir plus a simulant of Biejiajian pills were given for 48 weeks. The changes in liver stiffness measurement (LSM) and TCM syndrome scores before and after treatment were compared between the two groups to analyze the correlation. The data between groups were analyzed by t-test/Wilcoxon rank sum test or χ(2) test. Pearson correlation coefficient was used to analyze the correlation between TCM syndrome scores and LSM values. Results: After 48 weeks of treatment, the LSM values of the two groups were significantly lower than those of the baseline (P < 0.001), liver fibrosis was significantly improved, and the LSM values of the treatment group were lower than those of the control group [(8.67 ± 4.60) kPa and (10.13 ± 4.43) kPa, t = -2.011, P = 0.049]. After 48 weeks of treatment, the TCM syndrome scores of the two groups were significantly reduced compared with the baseline (P < 0.001), and the clinical symptoms were significantly relieved, and the total effective rates of the improvement of the TCM syndrome scores in the two groups were 74.19% and 72.97%, respectively, but the differences between the groups were not statistically significant (χ(2) = 0.013, P = 0.910). Correlation analysis showed that there was no obvious trend between TCM syndrome scores and LSM values. There were no serious adverse reactions associated with the drug during the observation period of this study. Conclusion: Based on antiviral treatment with entecavir, regardless of whether it is combined with the Biejiajian pill, it can effectively reduce the LSM value, improve liver fibrosis, reduce TCM syndrome scores, and alleviate symptoms in patients with chronic hepatitis B with liver fibrosis and blood stasis syndrome. Compared with entecavir alone, the combined Biejia pill has greater efficacy in improving liver fibrosis and a favorable safety profile, meriting its implementation and widespread application.

Clinical study on Sun's sequential therapy of allergic rhinitis with lung deficiency and cold syndrome / 国际中医中药杂志

Objective:To evaluate the efficacy of Sun's sequential therapy (SST) of Chinese Medicine on allergic rhinitis with lung deficiency and cold syndrome.Methods:A total of 60 AR patients with lung deficiency and cold syndrome in otolaryngology clinic of Guang'anmen Hospital, from January to July 2020, who met the inclusion criteria, were randomly divided into 2 groups according to the random number table method, with 30 patients in each group. The Traditional Chinese Medicine (TCM) group was treated with oral Yupingfeng Powder and Cang'erzi Powder and the SST group was treated with oral and nasal steaming of Yupingfeng Powder and Cang'erzi Powder. Both groups were treated for 14 days. The clinical symptoms were scored before and after treatment, and the Nasal mucosa eosinophil (EOS) count was graded by Sheldon method to evaluate the clinical efficacy.Results:Twenty five patients in the SST group and 28 in the TCM group were analyzed. The total effective rate was 88.0% (22/25) in the SST group and 89.3% (25/28) in the TCM group, and there was no significant difference between the two groups ( χ2=2.83, P=0.883). The scores of nasal obstruction, runny nose, sneeze and total score in the SST group were significantly lower than those in the TCM group ( P<0.01), and the difference of Nasal mucosa EOS grade in SST group (2.76±0.27 vs. 1.52±0.36) was significantly higher than that of the TCM group ( P=0.01). Conclusion:The SST of Chinese medicine can improve the symptoms of nasal congestion, runny nose and sneezing in AR patients with lung deficiency and cold syndrome, and reduce the distribution of Nasal mucosa EOS.

YU Tian-yuan's experience in the clinical application of Danzhong (CV 17) for mental illness / 中国针灸

To summarize YU Tian-yuan's experience of applying Danzhong (CV 17) for mental illness in acupuncture and tuina. YU Tian-yuan uses Danzhong (CV 17) alone or in combination with other acupoints to treat mental illnesses such as insomnia, palpitation and chest distress. Professor YU emphasizes 4 tips when treating diseases, nourishing the heart to tranquilize by light stimulation; regulating spirit by combined stimulation; leaving the acupoints and holding on the meridian for a wide range of stimulation; using rubbing and pushing manipulation in several directions for regulating qi to soothe the chest. And in clinical practice, formed a unique therapy to treat mental illness.

Review of chemical constituents,pharmacological effects and clinical applications of Shengyu Decoction and predictive analysis of its quality marker / 国际中医中药杂志

Shengyu Decoction is a classic prescription for supplementing qi and blood. It has the pharmacological effects of supplementing blood, protecting nerves and anti-oxidation. It is commonly used in clinics to treat orthopedic, gynecological and internal diseases. This paper summarizes and analyzes the chemical composition, pharmacological action and clinical application of Shengyu Decoction in recent years, and predicts and analyzes the Q-marker of Shengyu Decoction with reference to the "Five Principles" of quality marker. The results show that the six components of Mullein glycoside, ferulic acid, Chuanxiong lactone A, ginsenoside Rb1, Ginsenoside Rg1 and astragaloside Ⅳ can be used as the Q-marker for Shengyu decoction, providing a reference basis for establishing a complete quality evaluation system of Shengyu Decoction.

Intervention effects and mechanism of Compound yu ’e nasal drops on allergic rhinitis in rats / 中国药房

OBJECTIVE To study the intervention effects and mechanism of Compound yu ’e nasal drops on ovalbumin induced allergic rhinitis in rats . METHODS The allergic rhinitis model of rat was induced with ovalbumin . Model rats were randomly divided into model group ，triamcinolone acetonide group （positive control ，0.026 mg/kg），Compound yu ’e nasal drops high-dose，medium-dose and low -dose groups （134.4、67.2、33.6 mg/kg），12 rats in each group . Another blank control group was set. Except for blank control group ，the corresponding drugs were given by nasal drip twice a day for 14 days. One hour after last administration，the nasal symptom scores of rats were recorded ；the levels of serum immunoglobulin E （IgE），interleukin-2（IL- 2），IL-13 and tumor necrosis factor -α（TNF-α）were measured by enzyme -linked immunosorbent assay . The changes of nasal mucosa in rat were observed by HE staining . The expressions of TNF -α，IL-2 and IL -13 in nasal mucosa were detected by Western blot. RESULTS Compared with blank control group ，nasal symptom score and the levels of serum IgE ，IL-2，IL-13，TNF-α in model group were increased significantly （P＜0.01）；obvious pathological injury was found in nasal mucosa ，and the expressions of TNF -α，IL-2 and IL -13 protein were increased significantly （P＜0.01）. Compared with model group ，Compound yu ’e nasal drops significantly reduced the nasal symptom score ，the levels of serum IgE ，IL-2，IL-13，TNF-α to different extents ，improved pathological injury of nasal mucosa and significantly inhibited the expressions of TNF -α，IL-2 and IL -13 protein（P＜0.05 or P＜ 0.01）. CONCLUSIONS Compound yu ’e nasal drops play significant effects against allergic rhinitis in rats by regulating the balance of t ype 1 helper T cells/type 2 helper T cells ，balancing and inhibiting the secretion of inflammatory cytokines .

On the Time Value of Yu Weihan’s Spiritual Strength / 中国医学伦理学

Yu Weihan, academician of the Chinese Academy of Engineering, devoted his whole life to the prevention and control of endemic diseases and basically eradicated the epidemic of Keshan disease. Academician Yu Weihan’s struggle experience has left us with valuable spiritual wealth. His spiritual strength is manifested in many aspects, such as the spirit of bearing hardships first, the spirit of innovative daring to be the first, the spirit of craftsmen striving for perfection, the patriotic spirit of caring for the people, and the dedication of being indifferent to fame and wealth. His great medical feelings of bravely shouldering the mission, caring for the people and taking root in the grass-roots have forcefully answered the three important questions: what kind of doctor to be, who to be and how to be a good doctor in the learning and working career of doctors in the new era. He set an model for doctors in later generations and highlighted the value of this spiritual strength for the time.

The dynamic metabolic profile of Qi-Yu-San-Long decoction in rat urine using UPLC-QTOF-MSE coupled with a post-targeted screening strategy / 药物分析学报

Qi-Yu-San-Long decoction(QYSLD)is a traditional Chinese medicine that has been clinically used in the treatment of non-small-cell lung cancer(NSCLC)for more than 20 years.However,to date,metabolic-related studies on QYSLD have not been performed.In this study,a post-targeted screening strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight full infor-mation tandem mass spectrometry(UPLC-QTOF-MSE)was developed to identify QYSLD-related xeno-biotics in rat urine.The chemical compound database of QYSLD constituents was established from previous research,and metabolites related to these compounds were predicted in combination with their possible metabolic pathways.The metabolites were identified by extracted ion chromatograms using predicted m/z values as well as retention time,excimer ions,and fragmentation behavior.Overall,85 QYSLD-related xenobiotics(20 prototype compounds and 65 metabolites)were characterized from rat urine.The main metabolic reactions and elimination features of QYSLD included oxidation,reduction,decarboxylation,hydrolysis,demethylation,glucuronidation,sulfation,methylation,deglycosylation,acetylation,and associated combination reactions.Of the identified molecules,14 prototype compounds and 58 metabolites were slowly eliminated,thus accumulating in vivo over an extended period,while five prototypes and two metabolites were present in vivo for a short duration.Furthermore,one pro-totype and five metabolites underwent the process of"appearing-disappearing-reappearing"in vivo.Overall,the metabolic profile and characteristics of QYSLD in rat urine were determined,which is useful in elucidating the active components of the decoction in vivo,thus providing the basis for studying its mechanism of action.

Agentes comunitários de saúde e o trabalho com usuários de drogas: obstáculos e desfechos possíveis / Community health agents and work with drug users: possible obstacles and outcomes / Agentes de salud comunitaria y el trabajo con usuarios de drogas: posibles obstáculos y resultados

INTRODUÇÃO: Na Estratégia Saúde da Família (ESF), o trabalho do Agente Comunitário de Saúde (ACS) é ponto inicial de ancoragem das ações em saúde voltadas à comunidade. OBJETIVO: compreender o entendimento dos ACS em relação à abordagem da temática das drogas no território em que atuam. Métodos: Estudo qualitativo, realizado com 6 ACS de uma Equipe de Unidade de ESF. Definiu-se a amostra por exaustão. Foram realizadas entrevistas, posteriormente transcritas e organizadas conforme o Discurso do Sujeito Coletivo. RESULTADOS: Foram evidenciados entraves e equívocos no processo de trabalho dos ACS no manejo das demandas relacionadas ao uso de álcool e drogas. CONCLUSÃO: A percepção dos ACS sobre a temática do uso de drogas no cotidiano do serviço de saúde é cautelosa e restrita. Mesmo diante da identificação disto como um problema do território, suas intervenções ocorrem mediante manifestação voluntária do usuário, quando este busca o serviço de saúde.

INTRODUCTION: In the Family Health Strategy (FHS), the work of the Community Health Agent (CHA) is the starting point for anchoring health actions aimed at the community. OBJECTIVE: to comprehend the understanding of the CHA in relation to addressing the theme of drugs in the territory in which they operate. Methods: Qualitative study, carried out with 6 CHA from an FHS Unit Team. The sample was defined by exhaustion. Interviews were carried out, later transcribed and organized according to the Collective Subject Discourse. RESULTS: Obstacles and misunderstandings were evidenced in the work process of the CHA in handling the demands related to the use of alcohol and drugs. CONCLUSION: The perception of CHAs on the theme of drug use in the daily routine of the health service is cautious and restricted. Even with the identification of this as a territory problem, their interventions occur through voluntary manifestation of the user, once he seeks the health service.

INTRODUCCIÓN: En la Estrategia de Salud Familiar (ESF), el trabajo del Agente de Salud Comunitaria (ASC) es el punto de partida para anclar las acciones de salud dirigidas a la comunidad. OBJETIVO: comprender lo entendimiento de la ASC en relación con el tema de las drogas en el territorio en el que operan. Métodos: Estudio cualitativo, realizado con 6 ASC de un equipo de la Unidad ESF. La muestra se definió por agotamiento. Las entrevistas se llevaron a cabo, luego se transcribieron y se organizaron de acuerdo con el Discurso del Sujeto Colectivo. RESULTADOS: se evidenciaron obstáculos y malentendidos en el proceso de trabajo de la ASC en el manejo de las demandas relacionadas con el uso de alcohol y drogas. CONCLUSIÓN: La percepción de los ASC sobre el tema del consumo de drogas en la vida diaria del servicio de salud es cautelosa y restringida. Incluso con la identificación de esto como un problema del territorio, sus intervenciones ocurren a través de la manifestación voluntaria del usuario, cuando este busca el servicio de salud.

Mechanism of action of Xiayuxue decoction in inhibiting liver fibrosis by regulating glial cell line-derived neurotrophic factor / 临床肝胆病杂志

ObjectiveTo investigate whether Xiayuxue decoction exerts an anti-liver fibrosis effect by inhibiting glial cell line-derived neurotrophic factor (GDNF). MethodsA total of 24 C57BL/6 mice were randomly divided into control group, model group, and Xiayuxue decoction group. The mice in the model group and the Xiayuxue decoction group were given intraperitoneal injection of 10% CCl4, and those in the Xiayuxue decoction group were given 0.4678 g/kg Xiayuxue decoction by gavage since week 4. The liver function parameters alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured, and liver histopathology was observed. Immunohistochemistry was used to measure the protein expression of alpha-smooth muscle actin (α-SMA) and GDNF. GFP-Col-HSC and human primary hepatic stellate cells (HSCs) were treated with GDNF (10 ng/ml), and HSC activation was measured. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the control group, the model group had significant increases in the levels of ALT and AST, and compared with the model group, the Xiayuxue decoction group had significant reductions in the levels of ALT and AST (all P＜0.01). Liver histopathology showed that the model group had marked inflammatory cell infiltration and formation of fibrous septa by proliferated collagen fibers, and the Xiayuxue decoction group had loose fibrous septa and alleviated inflammatory cell infiltration. Immunohistochemistry showed that compared with the control group, the model group had significant increases in the expression of α-SMA and GDNF (both P＜0.01), which were observed in fibrous septa, and compared with the model group, the Xiayuxue decoction group had significant reductions in the expression of α-SMA and GDNF (both P＜0.05). Western blotting showed that the control group had relatively low expression of GDNF in liver tissue, the formation of liver fibrosis at week 6 of CCl4 modeling, and an around 10-fold increase in the expression of GDNF, and the Xiayuxue decoction group had significantly inhibited protein expression of GDNF (P＜0.01); there were significant increases in the expression of α-SMA and collagen type I α1 (Col1) in mice with liver fibrosis, with significant reductions in α-SMA and Col1 after treatment with Xiayuxue decoction (all P＜0.01). The in vitro experiment showed that GDNF induced the significant increases in the protein expression of α-SMA and Col1 in HSCs, which was significantly inhibited by Xiayuxue decoction (all P＜0.01). ConclusionThe expression of GDNF is significantly upregulated in the formation of liver fibrosis. GDNF can induce HSC activation, and Xiayuxue decoction can exert an anti-liver fibrosis effect by inhibiting GDNF.

Comparison of clinical effects of the Yu flap and the Karapandzic flap in repairing greater than 2/3 lower lip defects / 口腔疾病防治

Objective@#To compare the clinical effect of the Yu flap and the Karapandzic flap in repairing greater than 2/3 defects of the lower lip and to provide a reference for clinical application.@*Methods@#Ten patients with greater than 2/3 lower lip defects after surgical resection of lower lip tumors and vascular malformations were enrolled: 5 patients were repaired with the Yu flap (Yu flap group) and 5 patients were repaired with the Karapandzic flap (Karapandzic flap group). Follow-up for at least 1 year was conducted to evaluate the morphology (symmetry, stoma, exposure of vermilion) and function (sensory function, motor function) of the reconstructed lower lip.@*Results @#All the flaps survived, and all wounds showed primary healing. The lower lips reconstructed with the Yu flap or the Karapandzic flap obtained similar satisfactory oral function. The sensory function was essentially restored. There were no obvious obstacles in speech and expression, and no saliva leakage occurred. In the Yu flap group, only 1 patient had slight microstomia. In the Karapandzic flap group, 2 patients had slight microstomia and 3 patients had moderate microstomia. 90% (9/10) of the patients were very satisfied with the postoperative outcome, and 1 patient in the Karapandzic flap group was basically satisfied. @*Conclusion@#Both the Yu flap and the Karapandzic flap can be used to repair greater than 2/3 lower lip defects and reliable outcomes can be achieved. These two methods can achieve similar oral functions, but the effect of the Karapandzic flap is inferior to that of the Yu flap in terms of aesthetic appearance, and microstomia often occurs, while the Yu flap can generally maintain the original size of the mouth cleft.

Clinical efficacy,SAS and HAMD scale scores and 5-HT level of patients with post-stroke depression treated by awakening The Mind and opening the qi needle / 中风与神经疾病杂志

@#Objective　To explore the clinical effect,SAS and HAMD scale score and 5-HT level of patients with post-stroke depression treated by awakening the mind and opening the qi needle.Methods　A total of 93 patients with poststroke depression were included in the study,all of whom were admitted to our hospital from September 2018 to October 2020.Patients were divided into the control group and the observation group by random number table method.Patients in the control group (46 cases) were given conventional western medicine treatment.We compared between two groups of patients before and after treatment,treatment of depression self-rating scale (SDS) and Hamilton depression scale (HAMD) score changes,serotonin (5-HT) level change and neural function defect scale (NHISS) score and active ability of daily life (ADL) scale,simple mental state examination (MMSE) score changes,serum interleukin 6 (IL-6),interleukin 8 (IL-8),tumor necrosis factor alpha (TNF alpha) level changes,adverse reaction conditions.Results　The total effective rate (95.74%) in the observation group was higher than that in the control group (82.61%)(P<0.05).Before the treatment,two groups of patients with SDS,HAMD,5-HT,NHISS,ADL,MMSE,IL-6,IL-8 and TNF alpha index comparison(P>0.05),groups of patients after treatment,HAMD,SDS 5-HT,NHISS,ADL,MMSE,IL-6,IL-8,TNF alpha indexes are improved,such as observation group after treatment in patients with SDS,HAMD,5-HT,NHISS,ADL,MMSE,IL-6,IL-8 and TNF alpha indicators are superior to control group(P<0.05).The adverse reaction rate in the observation group (2.13%) was lower than that in the control group (15.22%)(P<0.05).Conclusion　The method of “awakening the mind and opening the depression” has a significant clinical effect on post-stroke depression.The patients’ depressive symptoms are improved and the recovery is good.Meanwhile,it can also reduce the side effects of medication in patients.

Effect of YU Ying-ao's Tongzhifang in Treatment of Epilepsy Based on Clinical Diagnosis and Treatment Data / 中国实验方剂学杂志

Objective:Analyze the composition of Tongzhifang，regularity of modified prescriptions based on outpatient medical records of national famous Chinese medicine practitioner Professor YU Ying-ao for the treatment of epilepsy as well as data mining， and summarize the clinical experience of Professor YU in diagnosis and treatment of epilepsy， in order to provide the reference basis for the traditional Chinese medicine treatment epilepsy. Method:Based on the data of outpatient medical records of Professor YU for epilepsy diagnosis and treatment，this study conducted standard patient follow-up，clinical data collection，pretreatment and mining analysis，and was confirmed through expert interviews. Liquorice 2.3 data mining platform and SPSS 17.0 were used for data management and statistical analysis. Result:A total of 1 219 medical records of 243 cases were completely collected. As a result， Professor YU Ying-ao's Tongzhifang for epilepsy was based on ancient prescription Baijinwan，in which Shenglongchi and Ostreae Concha enhance tranquilizing and hyperactivity-subduing effects，Citri Reticulatae Pericarpium，Armeniacae Semen Amarum，Arisaema Cum Bile and Bambusae Caulis in Taenias eliminate phlegm and free channels， Salviae Miltiorrhizae Radix et Rhizoma and Persicae Semen promote blood circulation to remove blood stasis， and the 10 herbs were meticulously composed and well compatible. According to the small sample data，the total effective rate of patients with tetany type epilepsy taking this prescription for 3 months and 6 months was 80% （P<0.01）， with no adverse reactions. As for the dosage of Tongzhifang，the dosage for adult pills is generally 3-6 times that of decoction prescriptions，and the dosage for children's pills is generally 5-10 times that of decoction prescriptions. Under the guidance of the combination of disease differentiation and syndrome differentiation，epilepsy at the acute phase was treated with additionally Hupomo 1.5-3 g，the ratio of Curcumae Radix to Alumen is usually 4∶1， severe convulsion was treated with additionally Uncariae Ramulus Cum Uncis， Bombyx Batryticatus and Pheretima， epilepsy due to trauma was treated with additionally Typhae Pollen， Eupolyphaga Steleophaga， Carthami Flos and Radix Paeoniae Rubra； constipation was treated with additionally Cannabis Fructus； fatigue was treated with additionally Astragali Radix； dyspepsia was treated with additionally Galli Gigerii Endothelium Corneum； and irritability treated with additionally Gentiana scabra Bunge. Conclusion:The composition of Professor YU's Tongzhifang for treating epilepsy with Qianzhen Zhixian Huatan Tongluo method is relatively stable. Modified prescriptions are based on syndrome differentiation. Clinical experience and dosage range of decoction prescription and pill prescription for adults and children are available.

Dynamic Changes of Trace Elements in Yu Salvia miltiorrhiza in Different Growth Periods / 中国实验方剂学杂志

Objective:To establish an atomic absorption spectrometry to determine the content of eight trace elements of Yu Salvia miltiorrhiza in different growth stages，namely K，Cu，Na，Mg，Fe，Zn，Ca and Mn. Method:Micro-digestion-atomic absorption spectrometry was used to determine the trace elements in the roots of Yu S. miltiorrhiza. The HPLC method was used to determine the content of active constituents of Yu S. miltiorrhiza. Principal component analysis and correlation analysis were used to evaluate the results. Result:The contents of trace elements in Yu S. miltiorrhiza in different growth periods were significantly different. Cu had a significant positive correlation with the growth period，while Zn，Ca and Mn had significant negative correlations with the growth period. The comprehensive score of Yu S. miltiorrhiza in December was the best. The content of index components was negatively correlated with Mn，Zn and Ca，and positively correlated with Cu，Fe and Na. In soil，Mg，Fe，Ca and Mn were correlated with Zn，Ca and Mn，while Mn was negatively correlated with Cu. The content of K and Mg in the crude drug increased gradually with the change of the growth period，and the overall score of annual Yu S. miltiorrhiza was the best. Conclusion:The change of trace elements in Yu S. miltiorrhiza in different periods has certain regularity. Trace elements in soil have impacts on trace elements in medicinal materials. Trace elements in medicinal materials are closely correlated with index components and quality of medicinal materials.

VSD technique combined with Ointment for the treatment of foot skin defects / 中国骨伤

OBJECTIVE@#To explore the clinical effect of vacuum sealing drainage (VSD) technique and Ointment in the treatment of foot skin defect.@*METHODS@#From November 2017 to April 2019, 21 patients (21 feet) with foot skin defect were treated with VSD technique and Ointment. There were 17 males and 4 females, aged from 24 to 60 years old with an average of (37.8±11.2) years, 9 cases caused by traffic accident injury, 6 cases by heavy injury, 6 cases by falling injury. The time from injury to operation was for 3 to 36 (8.6±7.2) h, and the area of foot skin defect was for 20.35 to 83.43(47.2±19.5) cm. All patients underwent debridement or phaseⅠtemporary fixation in emergency, and three-dimensional imagingof the foot was performed by using Mimics software, and the defect area was rendered. The quality of wound healing and complications were observed, and the clinical effect was evaluated by Maryland foot function score.@*RESULTS@#All the 21 patients were followed up for 7 to 17 (10.8±2.7) months. There was no infection or nonunion in all patients. At the final follow-up, the skin margin of the healing site grew tightly, the skin was elastic, the texture was tough, the appearance was no obvious carbuncle. The time of wound healing was for 18 to 63 (41.2±13.3) days. Eight patients underwent stageⅡfixation or/and fusion, and all incisions healed by stageⅠ. According to Maryland's foot scoring, 9 cases got excellent results, 11 good, and 1 middle.@*CONCLUSION@#VSD can effectively remove the necrotic tissue of the wound, provide a smooth drainage of the wound, combine with Ointment to prevent infection and promote the rapid growth of granulation tissue, whose whole treatment cycle was short, the wound healing site was highquality, the limb function was good, and the clinical effect was satisfactory.