Separation,culture and identification of neural stem cells from rat embryo / 第二军医大学学报

This study is to set up a method for separating,culturing and identifying neural stem cells from cortex in rat embryo,observing their characteristics of multipotentiality and differentiation in vitro. By using serum-free cell culturing and clone technology,the stem cells were isolated,cultured and passaged from cortex in 14-day old rat embryo. The expression of Nestin antigen of brain cells were investigated by immunocytochemistry and H-E staining. It proved that cells separated from cortex in rat embryo possessed the potential to form clones and express nestin special antigen,the differentiated cells could express special antigen of neurons,astrocytes,and oligodendrocytes,they were stem cells of central nervous system.