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Objective:To investigate the effects of Shenfu injection on cerebral oxygen balance and cerebral ischemia-reperfusion injury in patients undergoing valve replacement during cardiopulmonary bypass,in order to Explore its efficacy and find reasonable route of administration. Methods: 32 patients undergoing valve replacement,aged 25～54,with the heart function of 2～3 level,were randomly divided into four groups with eight in each. Patients in group E1 received SFI 1.5 ml/kg before blocking of the aorta;Patients in group E2 received SFI 1.5 ml/kg after opening of the aorta;Patients in group E3received SFI 0.75 ml/kg before blocking and after opening the aorta,too. Patients in group C received 0.9% NaCl. Blood samples were taken from internal jugular vein and arteria radialis after trachea cannula(T1),10 min after CPB(T2),cooling stability stage(T3),recovery temperature to 33℃(T4),the end of CPB(T5) and 1 hour after the end of CPB(T6)for blood gas analysis.Then,Plasma S100? protein concentration、MDA、Glu and the activity of SOD in jugular vein bulb were determined. Results: The CERO2,SjvO2 changes in Group E1,E2,E3 were stable,moreover,SjvO2 was significantly higher than that in group C (P 0.05),In group E3,SOD had the highest activity at T6(P
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BACKGOUND: Disruption of the blood-brain barrier (BBB) can alter the internal milieu and may increase the release of excitatory amino acid neurotransmitters or catecholamines, which may affect metabolic rate or coupling. This study was performed to evaluate whether disruption of BBB by unilateral intracarotid injection of hyperosmolar mannitol would alter oxygen supply/consumption balance in the ipsilateral cortex. METHODS: Rats were anesthetized with 1.4% isoflurane using mechanical ventilation via tracheostomy. 25% mannitol was administered at a rate of 0.25 mlxkg-1s-1 for 30 s through unilateral internal carotid artery. The BBB transfer coefficient (Ki) of 14C-alpha-aminoisobutyric acid was measured in one group (N=7) after administering mannitol. Regional cerebral blood flow (rCBF), regional arterial and venous O2 saturation and O2 consumption were measured in another group using a 14C-iodoantipyrine and microspectrophotometry (N=7). RESULTS: Vital signs were similar before and after administering mannitol. Ki was significantly higher in the ipsilateral cortex (IC) than in the contralateral cortex (CC), (22.3+/-8.4 vs 4.4+/-1.1 microliterxg-1min-1). rCBF was similar between IC (105+/-21 mlxg-1min-1) and the CC (93+/-20). Venous O2 saturation was lower in the IC (43+/-7%) than in the CC (55+/-4). O2 consumption was higher in the IC (9.6+/-3.0 mlx100 g-1min-1) than in the CC (6.7+/-1.5). CONCLUSIONS: Our data suggested that increasing permeability of the BBB increased cerebral O2 consumption and deteriorated cerebral oxygen balance.