Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells / 한국실험동물학회지

Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-alpha. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-alpha (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-alpha. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.

Effect of Different Doses Methylprednisolone Combined with Monosialotetrahexosyl Ganglioside on Experimental Spinal Cord Injury / 中国康复理论与实践

@#Objective To compare the treatment effect of different doses methylprednisolone(MP)combined with monosialotetrahexosyl ganglioside(GM-1)on experimental spinal cord injury.Methods Based on the duplication of compressed spinal cord animal model,36 rats were divided into three groups randomly:the first group with high dose of MP,the second with moderate dose of MP and GM-1,the third with low dose of MP and GM-1.The motor evoked potential(MEP)and the grades of BBB were detected on the 7th day and 14th day after injury.Results The peak latencies and the amplitudes of MEP and the BBB grades changed significantly after injury in the three groups.After treatment by different administration,the recovery of the two combined groups was more obvious than that of the high dose MP group,and the effect with the low dose of MP and GM-1 group was most significant.Conclusion The treatment effect of low dose MP combined with GM-1 is better than the other two therapies on experimental spinal cord injury.

Effects of methylprednisolone and ganglioside GM-1 on a spinal lesion: a functional analysis

OBJECTIVES: The pharmacological effects of methylprednisolone (MP) and ganglioside GM-1 on spinal injuries have been thoroughly investigated, but only a few studies have evaluated the interaction between these two drugs. METHODS: Twenty-four Wistar rats were subjected to contusive injury of the spinal cord produced by the NYU system. These animals were divided into four groups: group I was injected with MP; group II was injected with GM-1; group III was injected with MP together with GM-1; and group control received physiological serum. The animals were evaluated with regard to their recovery of locomotive function by means of the BBB test on the second, seventh and fourteenth days after receiving the contusive injury to the spinal cord. They were sacrificed on the fourteenth day. RESULTS: This study demonstrated that the MP and GM-1 groups presented functional results that were better than those of the control group, although the enhanced recovery of group II (GM-1) relative to the control group was not statistically significant (p>0.05). The most notable recovery of locomotive function was observed in the group that received MP alone (p<0.05). The group that received MP together with GM-1 presented results that were better than those of the control group (p<0.05). CONCLUSION: Administration of methylprednisolone alone or with GM-1 was shown to be effective for recovery of locomotive function. Combined administration of these drugs resulted in better outcomes than administration of methylprednisolone alone.

Clinical observation of treating acute cerebral infarction by edaravone combined with Ganglioside GM_1 / 中国实用内科杂志

Objective To evaluate collaborative effect of the combination with edaravone and ganglioside GM1 (GM1) in treatment of acute cerebral infarction.Methods Factorial design was adopted in the study.All 71 patients with acute cer- ebral infarction were divided into the general treatment group(treated routinely by Compound Salvia Mihiorrhiza Bunge In- jection and aspirin)18 eases,the edaravone group(treated by edaravone and the general treatment)17 case,the GM_1 group (treated by GM_1 and the general treatment)18 cases,the combined treatment group(treated by edaravone and GM1 and the general treatment)18 cases.NIHSS score and Barthel Index(BI) score were recorded before treatment and after treat- ment.Results edaravone and GM_1 reduced NIHSS score of patients with acute cerebral infarction respectively(edaravone :F=12.36,P

Protective effect of combined NGF and ganglioside GM_1 on hypoxic-ischemic brain injury of newborn rats / 第三军医大学学报

Objective To study the therapeutic effect of combined administration of nerve growth factor(NGF)and ganglioside GM_ 1 on the hypoxic-ischemic brain injury(HIBI)rat model and the relevant involving mechanisms.Methods A total of 120 7-day old rats were randomly divided into 5 groups:sham-operation group,HIBI group,NGF group,GM_ 1 group and NGF+GM_ 1 group.HE staining,immunohistochemical staining and TUNEL staining were employed respectively to study the morphological changes,bcl-2 and Bax expressions and apoptosis in the hippocampal neuron after treatment.Results Pathological changes were observed in the hippocampal neuron after HIBI.In NGF+GM_ 1 group,the level of Bcl-2 expression was increased significantly(P

Expressions of nitric oxide synthase in neonatal rat after hypoxia-ischemia and their changes while ganglioside GM_1 administrated / 中国临床药理学与治疗学

AIM: To study the neuroprotective effect and possible mechanism of ganglioside GM 1 on neonatal hypoxic-ischemic-encephalopathy(HIE). METHODS: A rat model of neonatal HIE was established, then the pathological changes and expressions of nitric oxide synthase (NOS) in the brain tissues were investigated in different periods after hypoxia-ischemia (HI) and the subseqent changes of the above results after GM 1 administrated. RESULTS: The damage of the brain exposed to HI were alleviated significantly after GM 1 administrated. The levels of NOS expressions in the brain tissue increased after HI. GM 1 could inhibit NOS expressions induced by HI. CONCLUSION: GM 1 may have some protective effects on neonatal HIE, and the possible mechanism is related to the partial inhibition of NOS expression.

Expressions of glutamate and EAAC1 glutamate transporter in neonatal rat after hypoxia-ischemia and their changes after administrated ganglioside GM1 / 中国临床药理学与治疗学

AIM: To study the neuroprotective effect and possible mechanism of ganglioside GM1 on neonatal rats with hypoxic ischemic encephalopathy (HIE). METHODS: A rat model of neonatal HIE was established, then the pathological changes and expressions of glutamate and EAAC1 glutamate transporter in the brain tissues were investigated in different periods after hypoxia ischemia (HI) and the subsequent changes of the above results after GM1 administrated were studied too. RESULTS: The damages of the brain by exposed to HI were alleviated remarkably after GM1 administrated. The levels of glutamate neuron expressions in the brain tissue decreased after HI but EAAC1 increased. GM1 could partly prevent glutamate neuron reduction induced by HI and increase EAAC1 expression. CONCLUSION: GM1 may have some protective effects on glutamate neuron in neonatal HIE, and the possible mechanism is related to the partial increasing of EAAC1 expression.