Research progress in reamer-irrigator-aspirator technique in the treatment of bone defects and intramedullary canal infection / 中华创伤杂志

Treatment of large bone defects and nonunion induced by various causes requires bone graft, and autologous iliac bone graft has always been considered as the gold standard for the treatment of bone transplantation. However, there exist problems such as postoperative pain and numbness in the donor area or insufficient bone taking. The application of reamer-irrigator-aspirator（RIA）technique can reduce complications in the donor area and collect a maximum of 90 ml of autologous bone to treat large bone defects. In addition, it can also be used to remove the infected lesion and residual bone cement in the medullary cavity. The authors review the instrument structure, operation process, clinical application and complications of RIA technique, so as to provide a reference in the treatment of bone defects and intramedullary canal infection.

Comparação da eficácia da escova interproximal manual e irrigador bucal tipo waterflosser na higiene periimplantar em pacientes reabilitados com prótese total fixa sobre implantes: ensaio clínico randomizado controlado cruzado / Comparison of oral irrigator and interproximal brush in peri-implant oral hygiene in implant-fixed full-arch mandibular prosthesis: randomized controlled crossover clinical trial

Dificuldades na higienização de próteses fixas implanto-suportadas é uma queixa frequente dos pacientes. Embora a higiene bucal seja primordial para a manutenção em longo prazo, não há consenso na literatura com relação à eficácia de protocolos de higienização em implantes dentários e as melhores abordagens ainda estão por ser estabelecidas. Assim, o objetivo do presente estudo foi avaliar e comparar a eficácia de 2 protocolos de higienização, irrigador bucal e escova interproximal, como adjuvantes à escovação convencional na limpeza peri-implantar entre indivíduos reabilitados com prótese total implanto-suportadas. Para tal, um ensaio clínico randomizado controlado cruzado foi realizado com 21 indivíduos. No momento da seleção, cada participante foi aleatoriamente designado para um dos grupos do estudo: 1) grupo IB = escova interproximal + escova convencional ou 2) grupo IO = irrigador oral + escova convencional. Após 60 dias sob o protocolo de tratamento inicial, e transcorridos um período de washout de 30 dias, todos os participantes mudaram seus respectivos protocolos de higiene bucal. Os desfechos presença de placa bacteriana peri-implantar (PPi) e sangramento à sondagem (SSi) foram coletados no baseline, 7, 14, 30 e 60 dias após o início de cada tratamento. A análise estatística incluiu comparações intra e intergrupos através do teste de Friedman. Além disso, uma regressão logística de efeitos mistos foi usada para determinar as variáveis preditoras associadas aos desfechos do estudo. Ambos os grupos apresentaram melhorias no controle de PPi e SSi, embora as reduções tenham sido maiores no grupo IO, em todos os tempos de avaliação. As melhorias no controle de PPi e SSi após o período de monitoramento de 60 dias foram, respectivamente, cerca de 36% e 33% maiores no grupo IO. Escovação interproximal e sítios linguais foram associados com a ocorrência de PPi e SSi nos modelos logísticos finais de efeitos mistos. Os achados do presente estudo suportam a recomendação do irrigador bucal como adjuvante a escovação convencional na higiene peri-implantar em indivíduos reabilitados com prótese total inferior implanto-suportada (ensaiosclinicos.gov.br #RBR-62hsp9).

Comparison of oral irrigator and interproximal brush in peri-implant oral hygiene in implant-fixed full-arch mandibular prosthesis: randomized controlled crossover clinical trial. Great difficulties in oral hygiene of implant-fixed prostheses are a common complaint of patients. Although oral hygiene is paramount for long-lasting healthy and functional prosthetic rehabilitations, there is no literature consensus with respect to the efficacy of self-performed oral hygiene protocols around dental implants and the best approaches are yet to be established. Therefore, the aim of the present study was to evaluate and compare the efficacy of oral irrigator and interproximal brush as adjuvant to conventional brushing in peri-implant oral hygiene among individuals rehabilitated with implant-fixed full-arch mandibular prosthesis. A randomized controlled crossover clinical trial was conducted with 21 individuals. At the enrollment, each participant was randomly assigned to one of the study groups: 1) IB group = interproximal brush + conventional brush ou 2) OI group = oral irrigator + conventional brush. After 60 days under the initially treatment and a 30-day washout period, all participants changed their respective oral hygiene protocol. Peri- implant dental plaque (PiDP) and bleeding on probing (BOPi) were collected at baseline, 7, 14, 30 and 60 days after the beginning of each treatment. Statistical analysis included intra-and intergroup comparisons through the Friedman test. Mixed-effects logistic regression was used to determine the effects of predictors associated to study outcomes. Both groups presented improvements in PiDP and BOPi control, although reductions were greater in the OI group, at all examination times. Improvements in PiDP and BOPi after the 60-day monitoring period were, respectively, around 36% and 33% greater in the OI group. Interproximal brushing and lingual sites were associated with PiDP and BOPi in the mixed effects final models. Findings support the recommendation of oral irrigator as adjuvant to conventional brushing in the peri-implant oral hygiene in implant-fixed full-arch rehabilitated individuals (ensaiosclinicos.gov.br #RBR-62hsp9).

Development of portable early debridement device / 医疗卫生装备

Objective To develop a portable jet-flow device for preliminary debridement of wound mainly in battlefield,which can also be used for the treatment of nuclear or chemical weapon injury at wartime and peacetime.Methods The device was composed of a dynamic system,a servo system and consumable accessories,and developed based on a pneumaticpower scheme.Composite power unit free of dependence on electric power transferred the pneumatic power to three kinds of disinfectants through the four-way pipes.Results The device with easy operation consumed a few disinfectant and completed debridement within 30 minutes,with infection avoided effectively.Conclusion The device gains advantages in low weight,compact structure,function and low cost,and can be used for early debridement in field conditions.

Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages

MSC-based therapy is providing a cure for degenerative diseases with unmet medical need and usually iliac crest bone marrow (ICBM) are being applied in clinics. Alternative sources, including adipose tissue and reamer/irrigator/ aspirator hold great potential for isolating MCSs. Here, we compared original MSCs features of adipose tissue (Ad-MSCs) and bone marrow of long-bone (RIA-MSCs) or iliac crest, and the expression of chemokine receptors (including CXCR4, CX3CR1, CXCR6, CXCR2, CCR1 and CCR7) in these three sources, which are important in the context of homing. We further investigated the role of SDF-1/CXCR4 axis as a key player in motility of different population of MSCs using Transwell migration assay. All cells exhibited typical MSCs characteristics. However, different MSCs sources expressed different levels of chemokine receptors. Generally, the expression of these chemokine receptors was decreased with increasing passage (P) number from 2 to 3. Interestingly, it was observed that the CXCR4 expression and migration capacity in Ad-MSCs is significantly higher than ICBM and RIA-MSCs in P2. Although our data showed that CXCR4 had highest expression in P2 Ad-MSCs, but it dramatically declined following sub-culturing in the P3. Hence, to improve homing of MSCs by means of chemokine/their receptors axis, the source of isolation and passage number should be considered for clinical applications.