Laser stimulation of the acupoint ‘Zusanli’ (ST.36) on the radiopharmaceutical biodistribution in Wistar rats.

Laser used to stimulate acupoints is called laser acupuncture (LA). It is generally believed that similar clinical responses to manual acupuncture can be achieved. Here we analysed the effects of the laser (904 nm) at the ‘Zusanli’ acupoint (ST.36) of the stomach meridian on the biodistribution of the radiopharmaceutical Na99mTcO4. Wistar rats were divided into control (CG) and experimental groups (EG). The EG were exposed daily to the laser (904 nm) at ST.36 with 1 joule/min (40 mW/cm2) for 1 min. The animals of the CG were not exposed to laser at all. On the 8th day after LA, the animals were sedated and Na99mTcO4 was administered. After 10 min, the animals were all sacrificed and the organs removed. The radioactivity was counted in each organ to calculate the percentage of radioactivity of the injected dose per gram (%ATI/ g). Comparison of the %ATI/g in EG and CG was performed by Mann-Whitney test. The %ATI/g was significantly increased in the thyroid due to the stimulation of the ST.36 by laser. It is possible to conclude that the stimulation of ST.36 does lead to biological phenomena that interfere with the metabolism of the thyroid.

Effect of Acupoint 830 nm Laser Irradiation on Skeletal Muscle SOD and MDA Contents in Exhaustive Exercise Rats / 上海针灸杂志

Objective To investigate the effect of acupoint 830 nm semiconductor laser irradiation on skeletal muscle SOD and MDA contents in exhaustive exercise rats.Methods Rats did exhaustive eccentric exercise by downhill treadmill running. Rats were randomized into a blank group (group A), a model group (group B), a pre-exercise semiconductor laser pre-irradiation group (group C), a post-exercise laser irradiation group (group D) and a pre- and post-exercise laser irradiation combination group (group E). Except the blank group, each of the other groups was again divided into 24 h and 48 h subgroups, and there were groups B1, B2, C1, C2, D1, D2, E1 and E2, 8 rats each. Group B rats did one exhaustive exercise by treadmill running. Group C rats received laser pre-irradiation for 7 days before they did exhaustive exercise. Group D rats received laser irradiation after the exercise. Group E rats received laser pre-irradiation for 7 days before treadmill running and combined laser irradiation after the exercise. Specimens were taken in groups B1, C1, D1 and E1 at 24 h after the exercise and in groups B2, C2, D2 and E2 at 48 h after the exercise. A 830 nm semiconductor laser transmitter was used for laser irradiation. Point Chengshan was selected in the rats. Skeletal muscle superoxide dismutase (SOD) and malondialdehyde (MDA) contents were measured in every group before and after treatment.Results There was a statistically significant difference in muscular MDA content between group B1, D1, B2 or C2 and group A (P<0.05) and between group D2 or E2 and group B2 (P<0.05). There was a statistically significant difference in muscular SOD content between group B1, C1, D1 or B2 and group A (P<0.05) and between group C2, D2 or E2 and group B2 (P<0.05).Conclusion Acupoint 830 nm semiconductor laser irradiation can restore unbalanced oxidation-reduction reactions.

Effects of acupoint laser irradiation on gastric acid secretion and NO content in chronic atrophic gastritis / 中华物理医学与康复杂志

Objective To study the effects of He-Ne laser irradiation on the pH value of gastric juice, the ultrastructure of gastric parietal cells and the content of nitric oxide ( NO ) in chronic atrophic gastritis ( CAG ) . Methods Sixty healthy male Wistar rats were randomly divided into a normal control group, a model group, a drug group, an acupuncture group and a laser acupoint group, each of 12. A CAG model was successfully intro-duced to all except those in the normal control group through the administration of sodium salicylate and alcohol, combined with fasting and water deprivation, running fatigue and other interventions. The rats of the drug, acupunc-ture and laser acupuncture groups were given medications, acupuncture at Zusanli acupoint and laser irradiation at Zusanli acupoint accordingly. After 14 days of treatment, all of the rats were executed and diluted gastric juice and gastric tissues were collected. NO content in serum was determined using a nitrate enzyme reduction method, the pH value of gastric juice was measured, and the ultrastructure of the gastric mucosa was observed using a transmis-sion electron microscope. Results The average pH of the gastric juice and the average serum NO content were both significantly higher in the model group than in the normal control group. In the model group the membrane structure of the parietal cells was absent or partly dissolved and the mitochondria were swollen. Drugs, acupuncture and laser irradiating at the Zusanli acupoint all corresponded with reduced pH and NO content, but the most signifi-cant improvements were observed in the laser acupuncture group. In the laser acupuncture group the membrane structure of parietal cells was complete, and the cytoplasmic organelles had nearly returned to normal. Conclusions Laser irradiation can promote recovery from CAG, increasing the secretion of gastric acid and de-creasing the serum level of NO. Both have a good curative effect for CAG. Laser irradiation deserves further clinical research and promotion.

Design of a dual wavelength multi-channel laser acupuncture instrument / 国际生物医学工程杂志

Objective To design a laser acupuncture instrument with dual wavelength and multi-channel,under the guidance of traditional Chinese medicine acupuncture theory and according to low-level laser induced effect in bio-tissues and compatibility characteristics of acupuncture points.Methods Using SCM and semiconductor laser,hardware circuitry was designed to achieve the basic functions of the instrument,and the corresponding PC software was developed.Results The laser acupuncture treatment for several acupuncture points could be carried out at the same time with the same or different laser parameters (wavelength,power density,continuous/pulse),and each channel could output 635 nm and 808 nm laser,with continuous adjustable power.Conclusions The dual wavelength multi-channel laser acupuncture instrument can meet the needs of compatibility during clinical acupuncture treatment and is easy to operate.

Therapeutic Observation of Electroacupuncture plus Topical Laser Treatment for Hyperplasia of Mammary Glands / 上海针灸杂志

ObjectiveTo observe the clinical efficacy ofelectroacupunctureplustopical laser treatmentin treating hyperplasia of mammary glands.MethodFifty-eight patients with hyperplasia of mammary glands were randomized into a treatment group of 30 cases and a control group of 28 cases. The treatment group was intervened byelectroacupunctureplustopical laser treatment, while the controlgroup was by oral administration of Ru Zeng Ning tablets. The intervention began from 15 d prior to the menstrual period. After 3 successive treatment courses, the clinical efficacies were compared, and the efficacies in treating three different patterns of mammary glands hyperplasia were also compared, i.e. qi-stagnation due to liver depression, accumulation of phlegm and stagnation, and imbalance of the Thoroughfare and Conception Vessels.ResultAccording to the comprehensive efficacy evaluation, the total effective rate was 86.7% in the treatment group versus 57.1% in the control group, and the difference was statistically significant (P0.05).ConclusionElectroacupunctureplustopical laser treatmentis effective in treating hyperplasia of mammary glands, especially for the type of qi-stagnation due to liver depression.

Observations on the Efficacy of Electroacupuncture plus Weak Laser Fat Dissolution Technique in Treating Female Patients with Simple Obesity and Overweight of Stomach Heat Dampness Stagnation Type / 上海针灸杂志

Objective To investigate the clinical efficacy of electroacupuncture plus weak laser fat dissolution technique in treating female patients with obesity and overweight of stomach heat dampness stagnation type. Methods Forty-seven female patients diagnosed with simple obesity and overweight of stomach heat dampness stagnation type were randomly allocated to a treatment group of 25 cases and a control group of 22 cases. The treatment group received electroacupuncture plus weak laser fat dissolution technique and the control group, weak laser fat dissolution technique alone. Body weight, body mass index (BMI), waist circumference, abdominal circumference, hip circumference, body fat percentage (F%), visceral fat index (VFI) were measured in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results There were statistically significant differences in body weight, BMI, waist circumference, abdominal circumference, hip circumference difference values between before and after one course of treatment, between after one and two courses of treatment and between after two and three courses of treatment in the treatment group compared with the control group (P<0.01,P<0.05). There were statistically significant differences in visceral fat index difference values between after one and two courses of treatment and between after two and three courses of treatment in the treatment group compared with the control group (P<0.05). The total efficacy rate was 92.0%in the treatment group and 36.4%in the control group; there was a statistically significant difference between the two groups (P<0.01). Conclusion Electroacupuncture plus weak laser fat dissolution technique is an effective way to treat female simple obesity of stomach heat dampness stagnation type.

Research progress on the mechanism and application of laser acupuncture and moxibustion / 国际生物医学工程杂志

This review introduces the mechanism and advantages of laser acupuncture and moxibustion, the biological effect of low-level laser, the characteristics of acupuncture point area and the experimental laws of biological effect.On this basis, the existing problems in clinical application of laser acupuncture and laser acupuncture apparatus are pointed out.The combination therapy method with various wavelength are also discussed, and future development directions in the study are prospected.

Therapeutic Observation of Acupuncture plus He-Ne Laser Irradiation for Cervical Spondylotic Radiculopathy / 上海针灸杂志

Objective To observe the clinical efficacy of acupuncture plus He-Ne laser irradiation in treating cervical spondylotic radiculopathy (CSR) and to provide evidence for optimizing the treatment protocol. Method Totally 120 CSR patients were randomized into group A, group B, and group C, 40 in each group. Group A was intervened by acupuncture plus He-Ne laser irradiation, group B was by acupuncture alone, and group C was by the laser irradiation alone. The CSR rating scale was adopted for observation before and after intervention, and the therapeutic efficacy was also evaluated.Result The CSR rating score was changed significantly in all of the three groups after intervention (P<0.05). The change of CSR rating score in group A was significantly different from that in both group B and C (P<0.05). The total effective rate was 97.5% in group A, versus 92.5% in group C and 80.0% in group C, and the total effective rate of group A was significantly different from that in both group B and C (P<0.05).Conclusion Acupuncture plus He-Ne laser irradiation is an effective method in treating CSR.

Treatment of Chronic Diarrhea with Point-irradiation of He-Ne Laser in 50 Cases / 针灸推拿医学（英文版）

To treat 50 cases of chronic diarrhea by laser point-radiation on Shenque (CV 8 ), Tianshu (ST25), Zusanli (ST 36) and Point Anti-diarrhea with low power He-Ne laser. The total effective rate was 96%.

Effect of Chinese Herbal and Western Medicines Combined with Acupoint Pulse-Laser Stimulation for Calculus of Upper Urinary Tract / 广州中医药大学学报

Objective To observe the therapeutic effect of Chinese herbal and western medicines(CHM & WM)combined with acupoint pulse-laser stimulation(APLS)for calculus of upper urinary tract.Methods Sixty cases of calculus of upper urinary tract were allocated to the treatment group(Group A,n=36)and the control group (Group B,n=24).Group A was treated with CHM & WM combined with APLS and Group B with CHM alone.Results The therapeutic effect rate was 94.4% and 75.0% in Group A and Group B respectively (P

Effects of electrical and laser acupuncture to the stellate ganglion on autonomic nervous system / 全日本鍼灸学会雑誌

Previously we reported that acupuncture to the stellate ganglion stimulates the parasympathetic nervous system as well as the sympathetic nervous system. We measured effects of laser acupuncture to the stellate ganglion (SGL) or electrical acupuncture to the same (SGA) on R-R intervals, deep tissue temperature and blood presure by using 45 patients; 12 in SGL-group and 33 in SGA-group.<br>Results are as follows; (1) Means of heart rate (HR) decreased with 2bpm in the SGL-group, 3bpm in the SGA-group. (2) Coefficient of variation (CV) of R-R intervals increased with 1% in the SGA-group only. (3) Deep tissue temperature of the ipsilateral anterior forearm of the stimulation did not increase significantly after SGA, while deep tissue temperature increased with 0.3-0.5°C after SGL. Deep tissue temperature of the contralateral anterior forearm showed no significant change in the both groups. (4) Systolic blood pressure increased with 2-4mmHg after SGA and 4-5mmHg after SGL, respectively.<br>It is suggested that SGL increases deep tissue temperature by affecting autonomic nervous system, but not directly.