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Magnetic cell sorting technology is a highly specific and rapid cell sorting technology using superparamagnetic nanocomposites for cell sorting, which is widely used in immunology, stem cytology, oncology, clinical medicine and other fields. Magnetic cell sorting technology is divided into positive isolation, negative isolation/untouched cell isolation, depletion, multi-step isolation and automated cell separation systems. In this review, we firstly give a brief introduction to the classification and application of magnetic cell sorting technology, then discuss several new techniques and challenges based on magnetic cell sorting in recent years, such as improving the sorting efficiency by improving the structure of magnetic materials and magnetic field structure. The necessity of biological evaluation of magnetic cell sorting products was emphatically analyzed. Through the biological evaluation, the advantages and disadvantages of magnetic cell sorting products can be understood, and the research and development ability could be improved. Therefore, 10 biological evaluation technical parameters related to magnetic cell sorting products were proposed: yield, purity, sterility, cytotoxicity, cell morphology, viability, light scattering characteristics of cells, fluorescent antibody labeling ability of cells, cell activation and cell proliferation. The 10 biological evaluation technical parameters play an important role in promoting the standardized application of magnetic cell sorting.
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Objective To investigate the anti-tumor pathway and the effect of recombinant E.coli. LLO/OVA in C57BL/6 mice. Methods Magnetic sorting of splenic CD11c, CD4+ and CD8+ T cell from immunized mice with E.coli. LLO/OVA and E.coli. OVA vaccination were performed. In addition, the lymphocytes mixed reaction and IL-2 and IFN-? in the supernatant were detected. The percentage of OVA257-264 SIINFEKL specific CD8+ T cell were checked by flow cytometry. B16-OVA melanoma protection and inhibition response in E.coli. vaccinated mice were compared. Results Compared to E.coli. OVA, E.coli. LLO/OVA-vaccinated splenic CD11c cells stimulated proliferation of autogenic CD4+ T cells and IL-2 production of these cells. CD11c cells induced autogenic CD8+T cells proliferation and IFN-? secretion. A great number of OVA257-264 SIINFEKL-specific splenic CD8+ T cells were induced and the number of lung tumor nodules was significantly reduced in E.coli. LLO/OVA vaccinated mice. Conclusion Recombinant LLO, as an effective adjuvant of E.coli. OVA, can induce murine splenic CD11c cells activation and play potential roles on CD4+ and CD8+ T cells proliferation and IL-2 and IFN-? secretion of these cells, induce more OVA-specific CD8+ T cells and stimulate stronger tumor inhibition in vivo of vaccinated C57BL/6 mice.
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Objective To investigate the expression of CD133 in human nasopharyngeal carcinoma cell line SUNE,and to detect proliferative and differential ability of CD133+ tumor cells in vitro,and to identify tumor-like stem cells in SUNE.Methods Immunocytochemical staining method and flow cytometry were used to detect the expression of putative tumor-initiated cell marker CD133 in nasopharyngeal carcinoma cell line SUNE,and the isolation technique with immunomagnetic beads was applied to purify CD133+ cells.The proliferative ability of CD133+ tumor cells in vitro was estimated by MTT assay,and the proliferative ability of CD133+,CD133-and control SUNE cells were statistically compared.Finally,the immunocytochemical staining method and flow cytometry were used to detect differential ability of CD133+ tumor cells in vitro.Results CD133 was positively expressed only in 0.35% of the cells in nasopharyngeal carcinoma cell line SUNE.In the serum-free medium RPM I1640,the UV optical density of the CD133+ tumor cells enriched with immunomagnetic beads was 0.317?0.007,0.370?0.002 and 0.558?0.004 at 3,5 and 7 days,respectively.Compared with CD133-cells and control SUNE cells,CD133+ cells showed increased proliferative capacity(P