Analysis on results of WBC count and differential count of hydrothorax and ascites by humoral mode of Sysmex XE-5000 automated hematology analyzer / 国际检验医学杂志

Objective To explore the clinical application of humoral mode of the Sysmex XE-5000 automatic blood cell analyzer in the count and differential count of hydrothorax and ascites white blood cell(WBC).Methods The 196 clinical specimens of hy-drothorax and ascites from the hospitalized patients were collected and conducted the WBC count and differential count by the hu-moral mode of the Sysmex XE-5000 automatic blood analyzer (streaming technology with semiconductor laser-instrument method) and the artificial microscope counting method (manual method).The specimens were divided into two groups according to the in-strument counting results,the group A:(0 -1 500)× 106/L and the group B:>1 500 ×10 6/L and the correlation between two methods was analyzed.Results The data analysis demonstrated that the two kinds of methods for detecting the WBC count and dif-ferential count of hydrothorax and ascites had good correlation,and the difference had no statistical significance (P >0.05).Conclu-sion The humoral mode of the Sysmex XE-5000 automatic blood cell analyzer has a good application value for detecting WBC count and differential count of hydrothorax and ascites.

Cascas de semente de cacau em chocolate em pó / Cocoa-seed shells in powdered chocolate

Foram analisadas 40 amostras de chocolate em pó, adquiridas no comércio da cidade de São Paulo, a fim de se determinar a porcentagem de cascas de semente de cacau, utilizando o método da contagem de vasos espiralados. Concluiu-se que todas as amostras continham cascas e que 47,50/0 das mesmas continha percentagem de cascas além dos limites tolerados por legislações internacionais. Foi strgerida modificação no Código Brasileiro de Alimentos a fim de permitir um limite de até 1,75% de cascas de semente de cacau em chocolate em pó. Essa tolerância poderia ser estendida à massa de cacau e a outros derivados do mesmo (AU).

THE RELATIONSHIP BETWEEN NT- AND NPY-NEURONS IN THE ARCUATE NUCLEUS A DOUBLE LABELING IMMUNOELECTRON MICROSCOPIC STUDY / 解剖学报

The distribution of neurotensin (NT) and neuropeptide Y (NPY) in the rat hypothalamic arcuate nucleus has been studied ultrastructurally by means of double labeling preembedding immunoelectron microscopic PAP technique. First, the NPY immunoreaction was demonstrated by chromogen DAB, and second, the NT immunoreaction was demonstrated by ammonium molybdate-TMB method. After being stabilized by DAB-cobalt chloride, the vibratome sections were processed for electron microscopic study. The results showed that in the arcuate nucleus the NPY immunoreactive products appeared as high electron-dense granular or flocculent materials deposited diffusely in the organelles and matrix of perikaryon, around the dendritic microtubules and axonic small clear vesicles. Whereas the NT immunoreactive products were dense needle- or mass-like deposits distributed dispersively in the perikaryon, dendrites and axon terminals. They can easily be distinguished although being intermingled together. The NPY-containing dendrites and axons formed synaptic connections with immuno-negative axon terminals, NT-containing somata and dendrites formed also synaptic conections with negative axon terminals. In addition, NPY-positive axon terminals formed symmetrical axodendritic synapses with NT-positive dendrites. The present results provided another new ultrastructural evidence for the peptidergic synaptic regulation of NT neurons in hypothalamus.