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Objective To observe the intervention effects of methylprednisolone (MPred) to acute lung injury(ALI) model of rats induced by oleic acid.Methods Thirty of Sprague-Dawley rats were randomly divided into three groups which were normal control group (NC,n =6),oleic acid model group (n =12),and MPred group (n =12).Rats were injected with oleic acid at dose of 0.1mg/ kg via caudal vein and then ALI model was established.The rats of NC group were injected with 0.1 mg/kg of normal saline instead of oleic acid.In NC group rats were sacrificed by blood collection at 6h after NS injection.Blood samples and tissues were collected and stored freezing.Samples of the other groups were collected at 2h and 6h after the last treatment.The observation indexes are histomorphology of lung tissue,the wet and dry weight of lung (W/D),index of quantitative assessment (IQA) score,partial pressure of oxygen in artery (PaO2),and SOD,MDA,MMP-9 and TIMP-1 levels in the blood plasma and lung tissue.Results Lung surface hyperemia relieved obviously and pink secretion from trachea of rats in MPred group decreased compared with oleic acid model group.In light microscope,compared with oleic acid model group,effusion of inflammatory cell in alveolar space of rats in MPred group eased.W/D of rats in oleic acid model group advanced obviously compared with that in NC group,W/D of rats in medrol group lowered obviously compared with that in oleic acid model group.IQA scores of rats in oleic acid model group advanced obviously compared with that in NC group,IQA score of rats in MPred group lowered obviously compared with that in oleic acid model group.PaO2 of rats in oleic acid model group lowered obviously compared with that in NC group,PaO2 of rats in MPred group advanced obviously compared with that in oleic acid model group.The level of SOD in plasma and lung tissue of rats in oleic acid model group lowered obviously compared with that in NC group,SOD level in plasma and lung tissue of rats in MPred group advanced obviously compared with that in oleic acid model group.The level of MDA in plasma and lung tissue of rats in oleic acid model group lowered obviously compared with that in NC group,MDA in plasma and lung tissue of rats in MPred group advanced obviously compared with that in oleic acid model group.Compared with the NC group,the level of MMP-9 in the plasma and lung tissue of oleic acid induced ALI rats increased and TIMP-1 levels decreased significantly.The level of MMP-9 in the plasma of rats decreased and TIMP-1 level increased significantly in MPred group at 2h and 6h.Conclusion MPred can improve lung pathological injury,increase PaO2 level,decrease lung W/D ratio and IQA scores by modulating the level or activity of the SOD and MDA and MMP-9 and TIMP-1 in the plasma and lung tissues.It is speculated that the effects of anti-inflammation and anti-exudation of sodium aescinate may due to affecting on oxidative stress response as well as the decomposition and remodeling of extracellular matrix during inflammatory lung injury of acute lung injury rats.
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@#ObjectiveTo investigate the effects of morroniside on super oxide dismutase (SOD) and neurons in rats cortex with focal cerebral ischemia/reperfusion. MethodsThe animal model was induced by middle cerebral artery occlusion with suture embolus, cerebral ischemia 30 min and reperfusion 3 d or 7 d. Vitamin E for the positive control. The content of SOD was detected with spectrophotometry and the nerve cells was observed with immunohistochemistry. ResultsCompared with model group, morroniside （270 mg/kg）increased the activity of SOD and the number of neurons （30 mg/kg, 90 mg/kg, 270 mg/kg） significantly. ConclusionMorroniside may have neuroprotective effect and increasing the activity of SOD in rats cortex.
RESUMO
Aim To observe the intervention effects of sodium aescinate on acute lung injury model of rats induced by oleate. Methods Fifty four male SD rats were randomly divided into five groups: normal control group, sodium aescinate control group (without oleate) , oleate model control group,medrol interventional group and sodium aescinate interventional group. Acute lung injury models of rats were made by injecting oleate (OA, 0. 1 ml · kg~(-1) ) through caudal veins, and then rats were observed and killed to detect correlated in-dice. The observation indice were the histomorphology of lung, the wet and dry weights of lung ( W/D), score of injury of lung under light microscope (IQA ) , partial pressure of oxygen in artery ( PaO_2) , the levels of SOD and MDA in blood plasma and lung tissue. Results ① Histomorphology of lung: Lung surface hyperemia relieved obviously and pink secretion from trachea of rats in sodium aescinate interventional group and medrol interventioal group decreased significantly compared with oleate model control group. Under light microscope , compared with oleate model control group, effusion of inflammatory cells in alveolar space of rats in sodium aescinate interventional group and medrol interventional group decreased. ② The wet and dry weights of lung ( W/D ) ; W/D of rats in oleate control model group increased obviously compared with those in normal control group, W/D of rats in sodium aescinate interventional group and medrol interventional group decreased obviously compared with those in oleate model control group. ③ Score of injury of lungs under light microscope (IQA) ; IQA of rats in oleate model control group advanced obviously compared with that in normal control group. IQA of rats in sodium aescinate interventional group and medrol interventional group lowered significantly compared with that in oleate model control group.④ Partial pressure of oxygen in artery (PaO_2) : PaO_2 of rats in oleate model control group lowered significantly compared with that in normal control group. PaO_2 of rats in sodium aescinate interventional group and medrol interventional group improved significantly compared with that in oleate model control group. ⑤ The levels of SOD and MDA in blood plasma and lung tissue:The levels of SOD in plasma and lung tissue of rats in oleate model control group lowered significantly compared with those in normal control group. SOD in plasma and lung tissue of rats in sodium aescinate in-terventional group and medrol interventional group increased significantly compared with that in oleate model control group. The levels of MDA in plasma and lung tissue of rats in oleate model control group lowered significantly compared with those in normal control group. MDA in plasma and lung tissue of rats in sodium aescinate interventional group and medrol interventional group increased significantly compared with that in oleate model control group. Conclusion Sodium aescinate can improve W/D, IQA and PaO_2 by adjusting oxidization of the acute lung injury model of rats, which may provide a possible path for treating acute lung injury in clinical practice.