Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes / 中国结合医学杂志

<p><b>OBJECTIVE</b>To study the expression level and role of apoptosis-associated speck-like protein containing a caspase recruitment domain (PYCARD) gene transcript variant mRNA in peripheral blood mononuclear cells (PBMCs) of primary gout (PG) patients with different Chinese medicine (CM) syndromes.</p><p><b>METHODS</b>The expressions of PYCARD gene transcript variant mRNA and interleukin-1β (IL-1β) mRNA in PBMCs were investigated in 96 PG patients with acute phase (APPG, 44 cases) and non-acute phase (NAPPG, 52 cases) and 30 healthy controls (HCs) by reverse transcription-polymerase chain reaction (PCR) and/or realtime quantitative PCR. PYCARD and nuclear factor-κB (p50) [NF-κB (p50)] protein was detected by Western blot in PBMCs respectively. IL-1β, IL-4 and IL-10 protein levels in plasma of HCs and PG patients were measured by enzyme-linked immuno sorbent assay.</p><p><b>RESULTS</b>The main CM syndromes in APPG patients were obstruction of dampness and heat syndrome (ODHS, 36.36%) and intermingled phlegm-blood stasis syndrome (IPBSS, 27.27%), while in NAPPG patients were Pi (Spleen)-deficiency induced dampness syndrome (PDIDS, 40.38%) and qi-blood deficiency syndrome (QBDS, 26.92%). It showed statistical significances of the expressions of PYCARD gene and its transcript variant mRNA, the protein of PYCARD and NF-κB (p50) and the plasma IL-1β, IL-4 and IL-10 in APPG, NAPPG, ODHS, IPBSS, PDIDS and QBDS groups, compared with the HC group respectively (P<0.05 or P<0.01). There were also significant differences of mRNA expressions of PYCARD-1 and PYCARD-2 as well as protein expressions of IL-1β, IL-4 and IL-10 among the 4 CM syndromes groups (P<0.05 or P<0.01). Correlation analysis showed positive correlation between the mRNA expressions of PYCARD-1 gene transcript variant and IL-1β in APPG patients (r=0.3088, P=0.0183).</p><p><b>CONCLUSION</b>PYCARD gene and its transcript variant may play a critical and regulative role in the inflflammatory response of PG patients with different phases and CM syndromes.</p>

Expression of survivin gene and main transcript variants mRNA in colorectal carcinomas / 重庆医科大学学报

Objective: To investigate the clinical significance of the expressions of survivin gene and its main transcript variants mRNA in colorectal carcinoma. Methods: The expression of total survivin,survivin-△Ex3,survivin-2B,survivin-3B,survivin 2?,and CEA CAM 5 mRNA in 39 cases colorecta1 cancer,adjacent tissues (2.5 cm from the cancer margin) and norma1 colorecta1 tissue (10 cm from the cancer margin) were detected by RT-PCR technique and semi-quantitative methods use gel imaging analytical system or SYBR GreenⅠreal-time fluorescent PCR. Results: The positive rates of total survivin,survivin-△Ex3,survivin-2B,survivin-3B,survivin2?, and CEA CAM5 mRNA in colorectal carcinoma and adjacent tissue were 100.0%,92.3%,92.3%,79.5%,87.2%,97.4%,and 38.5%,25.6%,28.2%,7.7%,12.8%,12.8%,showing significant difference in two tissues (P0.05). Several transcript variants mRNA levels were associated with some clinicopathologic parameters (P