Caracterización cinética de la respuesta de secreción salival producida por ácido cítrico: diferencias con pilocarpina / Kinetic assessment of salivary secretory response to citric acid, as compared with pilocarpine

Background: Induction of salivation is becoming increasingly popular in the assessment of salivary gland status. Various mechanical or pharmacological procedures are empirically used to produce salivation. Oral stimulation by citric acid (AC) is by far the most used sialagogue procedure. Aim: To characterize the salivary secretory response to AC solutions applied to the dorsolateral tongue surfaces. Subjects and methods: Young healthy women from the upper levels of a medical career (n=19) participated as volunteers. Salivary volume and UV-absorbing organic material in saliva from single subjects were measured after various protocols of topical stimulation by AC. Results: After a single stimulation by 1-8 percent AC the salivary flow rate peaked before 30 seconds and recovered the basal level earlier than 2 minutes. Repetitive stimulations at 30-sec intervals kept the flow rate at a maximum. After suspending these stimulations, basal flow rate was recovered before 2 minutes. Repetitive AC-stimulations at 8-min intervals produced a series of identical and independent secretory responses. The concentration of organic material in saliva remained unaltered after the various modes of stimulation. Thus, the profile of organic material secretion was always a direct expression of changes in salivary flow rate. In contrast to AC, the oral administration of the cholinergic agonist pilocarpine (PIL) produced a two-wave salivary response that as a whole lasted for about 30 minutes. In this case the volume and the amount of organic material were at least 10-fold the ones secreted in response to AC. Conclusions: AC provoked a rapid and short-lived salivary response that differs markedly from the one produced by other secretagogues, like pilocarpine

Análise pela microscopia eletrônica de varredura da limpeza das paredes dos canais radiculares segundo as substâncias irrigadoras de acordo com três diferentes técnicas / Analisys through the scanning electron microscopy of root canals walls cleaning as to the irrigation substances in accordance with three differents techniques

Buscou-se verificar in vitro a remoçäo de restos pulpares e magma dentinário das paredes dos canais radiculares, quando do emprego na irrigaçäo final de substâncias químicas segundo três diferentes técnicas. No P.Q.C. utilizou-se a técnica telescópica e hipoclorito de sódio a 1 por cento. A irrigaçäo final foi de 30 ml: Grupo I - 10ml de hipoclorito de sódio a 1 por cento + 10 ml de ácido cítrico a 10 por cento + 10ml de água destilada; Grupo II - 15ml de hipoclorito de sódio a 0,5 por cento + 15 ml de EDTA-T; Grupo III - 10 ml de hipoclorito de sódio a 5 por cento + 10 ml de água oxigenada a 3 por cento + 10 ml de hipoclorito de sódio a 5 por cento. A avaliaçäo pelo MEV fez-se pela média da leitura dos túbulos dentinários visíveis segundo 3 observadores de uma fotomicrografia de cada terço...