RESUMO
HIGHLIGHTS Production of lipid nanoemulsions (<100 nm) of industrial interest with low energy demand. The antioxidant properties of babassu oil have been improved and the nanoemulsions are not cytotoxic. Babassu oil is a food and medicinal product. The nanoemulsion is strategic for the developed of new antioxidants phytotherapeutics.
Abstract Background: Babassu oil is an extract from a Brazilian native coconut (Orbignya phalerata Martius) and is used both as a food and a medicinal product. Methods: we produced two babassu oil nanoemulsions and evaluated them regarding their nanoscopic stability, antioxidant activity and cytotoxicity.The nanoemulsions were characterized by Dynamic Light Scattering, and their stability was investigated for 120 days. The antioxidant activity was assessed by Spectroscopy Electron Paramagnetic Resonance, and the cytotoxicity was assessed by a colorimetric method (MTT) with the NIH/3T3 cell lineage. Results: the results showed nanoemulsions with average hydrodynamic diameter lower than 100 nm (p(0.001).and a polydispersity index of less than 0.3 (p(0.001), indicating monodisperse systems and good stability at room temperature. The exposure of nanoemulsions at varying pH revealed that the isoelectric point was at 3.0, and the images obtained by Transmission Electron Microscopy showed spherical droplets with a size 27 nm. The antioxidant activity showed that the babassu nanoemulsions exposed to free radicals had a better response when compared to the oil free samples. The cell viability assays showed low toxicity of the formulation with viability over 92% (p(0.05). Conclusion: babassu oil nanoformulations showed low polydispersity and kinetic stability with effective antioxidant action. Therefore, they can be promising for application in the food industry or as antioxidant phytotherapeutics.
Assuntos
Óleo de Palmeira/química , Nanotecnologia , Antioxidantes , Extratos Vegetais/química , Indústria Alimentícia , Citotoxinas , Microscopia Eletrônica de Transmissão , Emulsões , NanocompostosRESUMO
A hibridização entre E. oleifera x E. guineensis dá origem ao óleo de palma nomeado como BRS Manicoré que possui elevado conteúdo de compostos bioativos. Objetivou-se avaliar os aspectos físico-químicos do óleo de palma BRS Manicoré avaliando quatro amostras de óleo hibrido quanto aos aspectos físico-químicos por meio das análises de índice de acidez titulável, acidez em ácido oléico, atividade de água, teores de umidade e cinzas e análise de cor, os resultados obtidos foram submetidos à Análise de Variância (ANOVA). As amostras analisadas apresentaram valores de índice de acidez de acordo com o estabelecido pela legislação, já os valores de acidez por ácido oléico foram maiores em relação ao reportado na legislação para o óleo de palma africano. Foi possível concluir que o óleo de palma demonstrou boa qualidade quanto a sua composição e aos benefícios à saúde humana.