RESUMO
In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.
Assuntos
Animais , Feminino , Humanos , Camundongos , Abscesso/parasitologia , Proteínas do Capsídeo/genética , Clonagem Molecular , Modelos Animais de Doenças , Mudança da Fase de Leitura do Gene Ribossômico , Giardiavirus/classificação , Coreia (Geográfico) , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , RNA Polimerase Dependente de RNA/genética , RNA de Cadeia Dupla/genética , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência , Tricomoníase/virologia , Trichomonas vaginalis/genética , VirulênciaRESUMO
Human dirofilariasis caused by Dirofilaria repens D. repens is a common zoonosis in the Mediterranean countries and parts of South Asia. During the last decade, it has been reported from countries previously considered non-endemic. This is likely due to the increased awareness regarding Dirofilaria infection. In some such cases however, dirofilariasis correlated with the travel of the patient to the endemic areas. We present the case of a Saudi male who had D. repens infection in the subcutaneous tissues of the forearm. The patient had traveled to Iraq, Syria, United Arab Emirates and Kuwait in the last 2 years. However, the known Dirofilaria endemic countries were not visited. Any prior occurrence of the human dirofilariasis in the Kingdom of Saudi Arabia is not documented. Lack of epidemiological studies or incidence reports in the Arabian region precludes any factual evaluation of Dirofilaria prevalence, which requires increased awareness amongst health workers regarding its potential occurrence in the native population
Assuntos
Humanos , Masculino , Dirofilariose/diagnóstico , Dirofilaria/patogenicidade , Dermatopatias Parasitárias , Abscesso/parasitologiaRESUMO
Os autores isolaram e estudaram morfologicamente Lagochilascaris minor Leiper 1909, material obtido na cirurgia de um abscesso cervical direito de um menino de cor branca, com cinco anos de idade, procedente da cidade de Colorado, no município de Vilhema, em Rondonia-Brasil. É sugerida uma chave para o diagnóstico diferencial das espécies do gênero Lagochilascaris