'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation

The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.

Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea / 대한임상미생물학회지

Blastomycosis, endemic in North America, has been hardly reported in Korea. We describe laboratory experience in phenotypic and molecular identification of Blastomyces dermatitidis first isolated in Korea. The patient was a 45-year-old male with pulmonary blastomycosis mimicking pulmonary tuberculosis. Diagnosis was based on culture and dimorphism combined with DNA target sequencing of internal transcribed spacers (ITS) and D1/D2 regions.

A Case of Pulmonary Blastomycosis Mimicking Pulmonary Tuberculosis / 결핵및호흡기질환

Blastomyces dermatitidis is a dimorphic fungus that causes the systemic pyogranulomatous disease known as blastomycosis. Blastomycosis most often involves the lungs, skin, and may involve nearly every organ in the body. It is difficult, however, to diagnose blastomycosis in the early stage of pulmonary disease because clinical manifestations are varied from subclinical infection to acute respiratory distress syndrome. Since blastomycosis is often accompanied by granulomatous inflammation in histopathologic findings, differentiation from other etiologic diseases is important. We report a case of a 45-year-old male with pulmonary blastomycosis who had been misdiagnosed with tuberculosis for 3 months.

Pulmonary blastomycosis diagnosed in Hawai'i.

Blastomycosis, a fungal infection caused by Blastomyces dermatitidis, was once thought to be endemic only to the Central and Great Lakes regions of the United States of America. We present the first reported case series of patients documenting the diagnosis of blastomycosis in the Pacific region. In both cases, exposure to endemic areas was retrospectively identified.

A Case of Blastomycosis after Traveling around Non-Endemic Area / 결핵및호흡기질환

Blastomycosis is a systemic pyogranulomatous disease that is caused by a thermally dimorphic fungus, Blastomyces dermatitidis. it's the disease is endemic in the south-eastern and south central states of the USA, which border the Mississippi and Ohio Rivers, the mid-western states and Canadian provinces bordering the Great Lakes as well as in a small area of New York and Canada adjacent to the St. Lawrence River.1 We encountered a case of blastomycosis, representing as a pulmonary manifestation after traveling around a non-endemic area and report it with a brief review of the relevant literature.

Systemic Blastomycosis with Osseous Involvement of the Foot: A Case Report / 대한족부족관절학회지

We report a patient who presented with three months of foot pain, lytic navicular bone lesions in the foot, and a painless ipsilateral leg skin ulcer. Bone and skin biopsies revealed organisms compatible with Blastomyces. Systemic blastomycosis is very rare, especially with bone involvement in the foot.

Esofagite infecciosa: agentes etiológicos / Infectious esophagitis: etiological agents

O estudo caracteriza-se por uma revisäo da literatura abrangendo os diversos agentes etiológicos infecciosos causadores de esofagite, de forma descritiva. Säo enfatizadas as diferentes apresentações macroscópicas, à luz da endoscopia digestiva. Säo ainda citadas características anatomopatológicas, além de manifestações clínicas gerais

Inoculation of Lacazia loboi into the subcutaneous tissue of the hamster cheek pouch

The subcutaneous tissue of the hamster cheek pouch, a site of immunologic privilege, has been used to investigate the potential infectivity of different types of parasites. It has been demonstrated that the implantation of fragments of lesions induced by the fungus Lacazia loboi, the etiologic agent of Jorge Lobo's disease, into the subcutaneous tissue of the hamster cheek pouch resulted in parasite multiplication and dissemination to satellite lymph nodes16. Here we describe the evolution of lesions induced by the inoculation of the isolated fungus into this immunologically privileged site. The morphology of the inflammatory response and fungal viability and proliferation were evaluated. Inoculation of the fungus into the cheek pouch induced histiocytic granulomas with rare lymphocytes. Although fungal cells were detected for a period of up to 180 days in these lesions, the fungi lost viability after the first day of inoculation. In contrast, when the parasite was inoculated into the footpad, non-organized histiocytic lesions were observed. Langhan's giant cells, lymphocytes and fungal particles were observed in these lesions. Fungal viability was observed up to 60 days after inoculation and non-viable parasites were present in the persistent lesions up to 180 days post-inoculation. These data indicate that the subcutaneous tissue of the hamster cheek pouch is not a suitable site for the proliferation of Lacazia loboi when the fungus isolated from human tissues is tested.

Blastomyces dermatitidis: lesiones pulmonares y manifestaciones sistémicas: avances y perspectivas de investigación / Blastomyces dermatitidis: pulmonary lesions and systemic symptoms

La blastomicosis es una infección fúngica rara, pero importante, ocurre principalmente en América, Africa y la India. Las epidemias por exposición a una fuente común, incluyeron pacientes de ambos sexos y de todas las edades, sin embargo, los casos de blastomicosis endémica se registraron principalmente entre los adultos jóvenes o ya maduros, y fueron más frecuentes en los hombres que en las mujeres. La neumonía es la manifestación más común de la blastomicosis, y los pulmones son casi siempre la pùerta de entrada de la primoinfección. la piel, los huesos, la próstata y el sistema nervioso central son también atacados, en ese orden descendente de frecuencia. La anfotericina B es curativa, pero debido a los efectos colaterales tóxicos, se ha investigado el uso de otros medicamantos orales para la terapia. El itraconazol ha venido reemplazando a la anfotericina B, en el tratamiento de la enfermedad. No se ha demostrado la eficacia terapéutica del fluconazol. En los pacientes con blastomicosis del sistema nervioso central, la anfotericina B, en el tratamiemto de la enfermedad. No se ha demostrado la eficacia terapéutica del fluconazol. En los pacientes con blastomicosis del sistema nervioso central, la anfotericina B es el tratamiento de elección

Avaliação imunológica de pacientes portadores de doença de Jorge Lobo / Immunological Evaluation of patients with Jorge Lobo's disease

Os autores investigaram a resposta imune humoral e celular de 16 pacientes portadores de doença de Jorge Lobo, através das seguintes avaliações: pesquisa de crioglobulinas, proteinas C reativa, auto-anticorpos, quantificação das imunoglobulinas séricas e do componente C3 do complemento e realização de etstes cutâneos de hipersensibilidades tardia com antígenos inespecíficos. Os resultados revelaram níveis séricos elevados de imunoglobulinas, principalmente IgC, bem como elevação dos níveis de C3. A maioria dos pacientes não reagiu aos testes intradérmicos realizados. Em conjunto os resultados sugerem alteração da resposta imune humoral e depressão parcial da resposta imune celular.

North American blastomycosis. About one case

The authors report on a new fatal case of systemic North American blastomycosis. They emphasized the occurence of this mycotic disease in Morocco as well as in other Non American countries and stress the differential diagnosis especially with tuberculosis

Leveduroses humanas (Visão geral do assunto) / Human yeast infections (Overview of subject)

Os autores tratam neste trabalho das principais leveduroses humanas, encarando a necessidade de um estudo mais acurado dessas blastomicoes. Inicialmente tecem comentários ligeiros sobre a importância das leveduroses na patologia humana, citando numerosos trabalhos realizados no estrangeiro, sobre o assunto. No Capítulo seguinte apresentam uma classificação médica das leveduroses humanas, assim esquematizada: leveduroses tegumentares (cutaneomucosas) e levedurides; estomatite cremosa ou sapinho bucal; língua negra pilosa; vulvo-vaginites por leveduras; pneumomicoses por leveduras; levedurose generalizada; granuloma criptococcico; esprú (?). Sobre cada uma dessas leveduroses tecem comentários de ordem clínica e terapêutica. O 3° Capítulo é destinado ao estudo das principais provas de laboratório que devem ser praticadas no diagnóstico das leveduroses humanas. No 4° Capítulo os autores apresentam a orientação que seguem no estudo micológico das leveduras patogênicas (AU).