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1.
Genet. mol. res. (Online) ; 7(2): 549-558, 2008. ilus
Artigo em Inglês | LILACS | ID: lil-640982

RESUMO

Intrinsically bent DNA is an alternative conformation of the DNA molecule caused by the presence of dA/dT tracts, 2 to 6 bp long, in a helical turn phase DNA or with multiple intervals of 10 to 11 bp. Other than flexibility, intrinsic bending sites induce DNA curvature in particular chromosome regions such as replication origins and promoters. Intrinsically bent DNA sites are important in initiating DNA replication, and are sometimes found near to regions associated with the nuclear matrix. Many methods have been developed to localize bent sites, for example, circular permutation, computational analysis, and atomic force microscopy. This review discusses intrinsically bent DNA sites associated with replication origins and gene promoter regions in prokaryote and eukaryote cells. We also describe methods for identifying bent DNA sites for circular permutation and computational analysis.


Assuntos
Humanos , Animais , DNA , Conformação de Ácido Nucleico , Origem de Replicação/genética , Regiões Promotoras Genéticas/genética , Biologia Computacional , Simulação por Computador , Células Procarióticas/metabolismo , Genes , Modelos Biológicos , Replicação do DNA/fisiologia
2.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 535-6, 555, 2004.
Artigo em Inglês | WPRIM | ID: wpr-640982

RESUMO

The aim of this study was to express and purify human histydyl-tRNA synthetase related gene and to prepare its polyantibody. The open reading frame was amplified by PCR, and then recombined into prokaryotic expression vector pQE30 and transformed into E. coli M15 for expression. The expressed products were induced by IPTG after the reconstructed pQE30 was transferred into M15. After purified by Ni affinity chromatography, the product was identified to be a single band by SDS-PAGE. The rabbits were inoculated with purified products. High-titer polyantibody was successfully prepared. Highly-purified expression product and prepared polyantibody may provide a good basis for further study.


Assuntos
Anticorpos/genética , Anticorpos/imunologia , Escherichia coli/genética , Escherichia coli/metabolismo , Histidina-tRNA Ligase/biossíntese , Histidina-tRNA Ligase/genética , Histidina-tRNA Ligase/imunologia , Fases de Leitura Aberta/genética , Células Procarióticas/metabolismo
3.
Experimental & Molecular Medicine ; : 53-59, 1999.
Artigo em Inglês | WPRIM | ID: wpr-56738

RESUMO

Although superoxide anions (O2.-) and H2O2 are generally considered to be toxic by-products of respiration, recent evidence suggests that the production of these reactive oxygen species (ROS) might be an integral component of membrane receptor signaling. In mammalian cells, a variety of extracellular stimuli have recently been shown to induce a transient increase in the intracellular concentration of ROS, and specific inhibition of the ROS generation resulted in a complete blockage of stimulant-dependent signaling. In the next few years, therefore, a flurry of research activity is expected in relation to the elucidation of ROS production in response to receptor stimulation, identification of ROS target molecules, and investigation of ROS elimination. The goal of this report is to review our current knowledge of ROS-regulated signal transduction and propose future directions.


Assuntos
Humanos , Animais , Células Eucarióticas/metabolismo , Peróxido de Hidrogênio/metabolismo , Oxirredução , Células Procarióticas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais
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