Determination of isofraxidin and astilbin by HPLC in rat plasma and its application after orally administration the extract of Sarcandra glabra

Sarcandra glaber is a common traditional Chinese medicine used to treat psoriasis and other infectious diseases, isofraxidin and astilbin are the main components of it. In order to study the pharmacokinetics of Sarcandra glabra, an HPLC method for simultaneous determination of isofraxidin and astilbin in rat plasma was established. Plasma samples were prepared using solid phase extraction method. C18 column with a guard was used, mobile phase was consisted of A [methanol] and B[0.1% aqueous acetic acid] with gradient elution as follows: 0 4min, A: 35%, B: 65%; 4 l[O]min, A: 35% 45%, B: 65% 55%; 10 20min, A: 45%, B: 55%. The flow rate was 1.2 mL/min from 0 to 4 min, 1.0 mL/min from 4 to 20 min. The detection wavelength was 300 nm. A linear correlation between drug amount and peak area was established for isofraxidin in the range of 20-320 ng and for astilbin in the range of 19-304 ng. The recovery was over 68% for both compounds, the accuracy was within 8%, and the inter-day and intra-day precisions were all less than 8%. The pharmacokinetics of isofraxidin and astilbin was studied after orally administration the extract of Sarcandra glabra

Spectrophotometric determination of thulium [lll] and dioxouranium ions using 8- [arylazo] -6,7 dihydroxy - 4 - methylcoumarin derivatives

The complexes of Tm [III] and UO2 [VI] with 8-[arylazo] -6,7-dihydroxy-4-methylcoumarin derivatives were investigated spectrophotometrically. The optimum conditions for chelation, pH, sequence of addition, time, solvents and stoichiometry were critically evaluated. The studies revealed that 1: 1 and 1: 2 [M: L] complexes were formed. The higher stability constants for Tm [III] complexes compared to those of UO2+ 2 complexes were explained in terms of the hydrolysis, steric effect and nature of bonding of both metal ions. Beer's Law was valid up to 16.89 mug/cm3 for Tm3+ complexes and 23.28 mug/cm3 for UO2+ 2 complexes. Spectrophotometric methods for the determination of Tm [III] and UO2+ 2 were presented. The methods were precise as shown by low standard deviations ranging from 0.0011 to 0.0146 and high correlation coefficient [r] ranging from 0.9965 to 0.9994. The values of molar absorptivity, epsilon [LM-1 cm-1] specific absorptivity, a [ml g-1 cm-1] and Sandell sensitivity and S [mug cm-1] indicated the method to be quite sensitive compared to recommended methods using arsenazo I and thoron. A photometric titration method using EDTA as titrant was given

Novel coumarins of potential adrenergic antagonistic activity

A series of 1-[8-methyl-4-phenyl-coumarin-7-yloxy]-3-[substituted amino] propan-2-ol [10-18], 1-[substituted coumarinyloxy]-3-[substituted-amino] propane [19-35], and 6 or 7 [[3.t.butyl-5-oxazolidinyl] methoxy substituted coumarins [36-39] were prepared to study the effect of change of structure on the beta- adrenergic antagonistic activity. The preliminary pharmacological tests were carried out, the results were given and a conclusion was drawn

Synthesis of some new coumarin derivatives incorporated to heterocyclie aromatic rings

3-bromo-4-methyl-7-coumarinyloxy acetic acid hydrazide was condensed with some aromatic aldehydes to give Schiff's bases 5 a-e which cyclized to thiazolidine derivatives 6a-e. Also, 7-O-[N-[succinimido] acetamido]-and 7-O-[[2-oxy-3-indolinylidene] acetic acid hydrazido]-3-bromo-4-methyl coumarins [3] and [4] were prepared. 7-O-[N-[sulfa drug] carbonyl methoxy]-3-bromo-4-methylcoumarin derivatives 10a-f were prepared via the formation of the acid chloride 9. Some of the new coumarin derivatives showed satisfactory results as molluscicidal activity against Biomphalaria alexandrina snails

Synthesis of some coumarin derivatives of possible biological activity

A series of bromocoumarins incorporated with aromatic derivatives via amino and ether linkages were synthesized. Also, hydrazocoumarin incorporated with thiosemicarbazides were prepared. Azole ring systems as 1,3,4-thiadiazole and 1,2,4-triazole were synthesized. Schiff bases which were cyclized with thioglycolic acid were prepared. Isatin moiety attached to hydrazocoumarin was prepared. The biological activity of some newly prepared compounds were carried out