RESUMO
Géis de fase cúbica de monoleína e água têm sido propostos como sistemas de liberação de fármacos com diferentes características, incluindo moléculas protéicas e peptídicas. O presente trabalho estudou a incorporação de lactoferrina, usada como molécula protéica modelo em várias concentrações em géis de fase cúbica de monoleína e água. A influência da lactoferrina nos géis de monoleína/água foi avaliada por microscopia de luz polarizada e estudo de suas propriedades reológicas, asssim como estudos de liberação in vitro foram realizados. A fase cúbica foi observada na presença da lactoferrina sendo que esta proteína não modificou as transições de fase dos géis, quando observados ao microscópio de luz polarizada...
Assuntos
Géis/farmacocinética , Técnicas In Vitro , Lactoferrina , Lipídeos/biossíntese , Preparações de Ação Retardada/farmacocinética , Preparações Farmacêuticas/análise , Microscopia de Polarização/métodos , Química Farmacêutica/métodos , ReologiaRESUMO
Tenoxicam transparent oil-water [TOW] gels were prepared using different oils; namely, isopropyl myristate, liquid paraffin, and myritol 318. The emulsifying agents used were Eumulgin B3, Cetiol HE, Brij 96, and Tween 80. The prepared gels were examined visually, microscopically and were photomicrographed. Conductivity, pH as well as rheological properties were determined. In vitro release studies, accelerated stability testing, and determination of anti-inflammatory activity were also performed. The results revealed that tenoxicam TOW gel prepared with liquid paraffin, Eumulgin B3, Cetiol HE combination and water [formula IV] was the most stable formula. It also affords reasonable pH, conductivity, rheological properties and in vitro drug release. The selected formula showed appreciable anti-inflammatory activity [determined by the Carrageenan induced rat paw edema] compared with a commercial piroxicam gel
Assuntos
Animais de Laboratório , Piroxicam/farmacologia , Géis/farmacocinética , Administração Tópica/métodosRESUMO
The release of piroxicam from different gel bases viz., Pluronic F127, methyl cellulose [MC], Carbopol 934, hydroxypropyl methyl cellulose [HPMC], Eudispert [medium viscosity [mv]] was determined and compared with its release organogel bases which are mixtures of Eudragit L 100 or S100 with glycerol or propylene glycol. Results showed that Pluronic F127 and HPMC gels were the most efficient bases, while MC and organogel of Eudragit showed the least results. The effect of different enhancers viz., polysorbate 80, dimethylformamide [DMF] and lecithin on the release pattern of the drug from Eudispert [mv], Carbopol 934 and MC gel bases was also studied using different concentrations of the enhancers. It was found that polysorbate 80 was the most efficient enhancer at concentration 5% w/w of the base. The prepared piroxicam gel which showed the highest amount of drug released were applied on the rat hind paw previously injected with carrageenan and compared with oral and intraperitoneally [i.p.] formulae. It was found that there was a significant inhibition in edema volume in all groups of the dosed rates