Glucose release by the liver under conditions of reduced activity of glucose 6-phosphatase

An inhibitor of glucose 6-phosphatase, isosteviol, was used in liver perfusion experiments to obtain a rough estimate of the control strength of the enzyme. Isosteviol only inhibited glucose release at high concentrations (1 mM), well above that needed for half-maximal action (70 micrnM). The decrease in glucose release was followed by an increase in the intracellular glucose 6-phosphate concentration. It was concluded that the control strength of glucose-6-phosphatase is relatively low