RESUMO
This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing. Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.
Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Grupo Borrelia Burgdorferi/classificação , Grupo Borrelia Burgdorferi/isolamento & purificação , Doenças Transmissíveis Emergentes/microbiologia , Doença de Lyme/microbiologia , Sequência de Bases , Brasil/epidemiologia , Doenças Transmissíveis Emergentes/epidemiologia , DNA Bacteriano/química , DNA Bacteriano/genética , Doença de Lyme/epidemiologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , População Rural , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.