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Braz. j. med. biol. res ; 24(5): 441-8, 1991. tab
Artigo em Inglês | LILACS | ID: lil-99475

RESUMO

Since dot-ELISA has recently been reported to be a sensitive, simple and method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for a antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. The sensitive of the techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93,4%). Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. These data indicate that dot-ELISA has a high spedificity and can be a useful tool in field evaluation


Assuntos
Humanos , Antígenos de Bactérias/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Glicolipídeos/imunologia , Immunoblotting/métodos , Imunoglobulina M/análise , Hanseníase/diagnóstico , Hanseníase Dimorfa/diagnóstico , Hanseníase Dimorfa/imunologia , Hanseníase Dimorfa/transmissão , Hanseníase Virchowiana/diagnóstico , Hanseníase Virchowiana/imunologia , Hanseníase Virchowiana/transmissão , Hanseníase Tuberculoide/diagnóstico , Hanseníase Tuberculoide/imunologia , Hanseníase Tuberculoide/transmissão , Hanseníase/imunologia , Hanseníase/transmissão , Valor Preditivo dos Testes , Pele/imunologia
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