Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
1.
Natural Product Sciences ; : 317-325, 2019.
Artigo em Inglês | WPRIM | ID: wpr-786428

RESUMO

Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC₅₀ value of 7.08 µg/mL) and displayed effects on T cell activation at a concentration of 400 µg/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH₂Cl₂-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 – 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC₅₀ values of 2.28 and 65.57 µM, respectively.


Assuntos
Ginsenosídeos , Interleucina-2 , Óxido Nítrico , Panax notoginseng , Panax , Plantas , Polímero Poliacetilênico , Saponinas , Linfócitos T
2.
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (6): 1259-1266
em Inglês | IMEMR | ID: emr-148561

RESUMO

Eclipta alba is a small branched perennial herb, which has been used as a traditional medicine in different countries mainly in tropical and subtropical regions of the world. The plant E. alba plays a significant role in the ayurvedic, traditional and unani systems of medicine. It is popularly known as "Bhringaraj". The herb has been known for its medicinal value and has been used as an analgesic, antimytotoxic, antihepatotoxic, antibacterial, antioxidant, antihaemorrhagic, antihyperglycemic and immunomodulatory and also recognized as a reincarnated plant. Broad range of chemical constituents have been detached from E. alba including coumestans, alkaloids, thiopenes, flavonoids, polyacetylenes, triterpenes and their glycosides. Pharmacological activities have been seen in the metabolites and extracts of this plant. Therefore this herb produces robust curative lead compounds, which would be propitious for humanity. The purpose of this review recapitulates all data related to E. alba considering its prodigious medicinal importance


Assuntos
Plantas Medicinais , Cumarínicos , Alcaloides , Saponinas , Triterpenos , Flavonoides , Polímero Poliacetilênico , Extratos Vegetais
3.
Electron. j. biotechnol ; 15(1): 4-4, Jan. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-640530

RESUMO

The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for “real-time” detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful environmental effectors. However, its actual application in the field has been complicated because lipid vesicles are unable to respond specifically to environmental changes. In this paper, we report several experimental trials leading to improved response in the detection of flagellated pathogens in drinking water. Chromatic biomimetic membranes of TRCDA/DMPC and TRCDA/DMPC/Tryptophan were used in agar and liquid media, which were challenged with different amounts of Escherichia coli and Salmonella typhimurium. In addition, the effect of some divalent cations on the interaction with vesicles TRCDA/DMPC was investigated. The results indicated an improvement in the response times, both visually and quantitatively, through the use of TRIS-EDTA and proper growing conditions for E. coli and Salmonella. With the application of both conditions, it was possible by incubation at 35ºC to promote bacterial growth, therefore avoiding a dramatic effect on the colour change over control samples which may invalidate the test. Our experiments indicated that the minimum bacterial concentration necessary to produce the transition from blue to red on the vesicles as biosensor approaches 10(8) CFU/ml within 4 hrs...


Assuntos
Água Potável/microbiologia , Técnicas Biossensoriais , Bactérias/isolamento & purificação , Membranas Artificiais , Polímero Poliacetilênico/química , Polímeros/química , Colorimetria , Microbiologia da Água
4.
Immune Network ; : 213-216, 2012.
Artigo em Inglês | WPRIM | ID: wpr-58013

RESUMO

Our previous report showed that polyacetylene compound, 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var. ussuriense has anti-inflammatory activity. In this study we investigated the role of the PA as inhibitor of caspase-1, which converts prointerleukin-1beta (proIL-1beta) to active IL-1beta and is activated by inflammasome involved in the inflammatory process. We tested the effect of PA on the production of pro-inflammatory cytokines, IL-1beta in murine macrophage cell line, RAW264.7. PA inhibited lipopolysaccharide (LPS)-induced IL-1beta production by macrophages at a dose dependent manner. PA also suppressed the activation of caspase-1. The mRNA level of ASC (apoptosis-associated spec-like protein containing a CARD), an important adaptor protein of inflammasome, was decreased in the PA treated group. Therefore our results suggest that the anti-inflammatory effect of PA is due to inhibit the caspase-1 activation.


Assuntos
Linhagem Celular , Cirsium , Citocinas , Macrófagos , Polímero Poliacetilênico , RNA Mensageiro
5.
China Journal of Chinese Materia Medica ; (24): 560-563, 2009.
Artigo em Chinês | WPRIM | ID: wpr-265383

RESUMO

<p><b>OBJECTIVE</b>To develop a green and rapid method for extraction of lobetyolin from C. pilosula.</p><p><b>METHOD</b>Extraction of lobetyolin from C. pilosula with supercritical carbon dioxide in the presence of ethanol was studied. The effects of pressure, temperature, volume of cosolvent and extraction time on efficiency and their interactive relationships were discussed, based on central composite design and response surface methodology (RSM).</p><p><b>RESULT</b>The key effect factor was volume of cosolvent. The extraction yield of lobetyolin was 0.078 6 mg x g(-1) when C. pilosula (40-60 mesh) was extracted at 30 MPa, 60 degrees C and 2 L x min(-1) (as CO2 in normal pressure and temperature) for 100 minutes with supercritical CO2 and 1 mL x min(-1) ethanol as dynamic cosolvent.</p><p><b>CONCLUSION</b>This result is better than that obtained from traditional method. Therefore, the optimized process is valuable for extraction of lobetyolin from C. pilosula.</p>


Assuntos
Dióxido de Carbono , Química , Fracionamento Químico , Métodos , Codonopsis , Química , Medicamentos de Ervas Chinesas , Química , Etanol , Química , Polímero Poliacetilênico , Química
6.
China Journal of Chinese Materia Medica ; (24): 2133-2135, 2008.
Artigo em Chinês | WPRIM | ID: wpr-252182

RESUMO

<p><b>OBJECTIVE</b>To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas.</p><p><b>METHOD</b>A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm.</p><p><b>RESULT</b>Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1).</p><p><b>CONCLUSION</b>The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.</p>


Assuntos
China , Cromatografia Líquida de Alta Pressão , Métodos , Codonopsis , Química , Medicamentos de Ervas Chinesas , Química , Raízes de Plantas , Química , Polímero Poliacetilênico , Controle de Qualidade
7.
China Journal of Chinese Materia Medica ; (24): 744-746, 2006.
Artigo em Chinês | WPRIM | ID: wpr-351766

RESUMO

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.</p><p><b>METHOD</b>C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.</p><p><b>RESULT</b>Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.</p><p><b>CONCLUSION</b>A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.</p>


Assuntos
Cromatografia Líquida de Alta Pressão , Métodos , Lobelia , Química , Estrutura Molecular , Plantas Medicinais , Química , Polímero Poliacetilênico , Química , Controle de Qualidade , Reprodutibilidade dos Testes
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA