Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Adicionar filtros








Intervalo de ano
1.
Experimental & Molecular Medicine ; : e147-2015.
Artigo em Inglês | WPRIM | ID: wpr-57308

RESUMO

Mammalian cells remove misfolded proteins using various proteolytic systems, including the ubiquitin (Ub)-proteasome system (UPS), chaperone mediated autophagy (CMA) and macroautophagy. The majority of misfolded proteins are degraded by the UPS, in which Ub-conjugated substrates are deubiquitinated, unfolded and cleaved into small peptides when passing through the narrow chamber of the proteasome. The substrates that expose a specific degradation signal, the KFERQ sequence motif, can be delivered to and degraded in lysosomes via the CMA. Aggregation-prone substrates resistant to both the UPS and the CMA can be degraded by macroautophagy, in which cargoes are segregated into autophagosomes before degradation by lysosomal hydrolases. Although most misfolded and aggregated proteins in the human proteome can be degraded by cellular protein quality control, some native and mutant proteins prone to aggregation into beta-sheet-enriched oligomers are resistant to all known proteolytic pathways and can thus grow into inclusion bodies or extracellular plaques. The accumulation of protease-resistant misfolded and aggregated proteins is a common mechanism underlying protein misfolding disorders, including neurodegenerative diseases such as Huntington's disease (HD), Alzheimer's disease (AD), Parkinson's disease (PD), prion diseases and Amyotrophic Lateral Sclerosis (ALS). In this review, we provide an overview of the proteolytic pathways in neurons, with an emphasis on the UPS, CMA and macroautophagy, and discuss the role of protein quality control in the degradation of pathogenic proteins in neurodegenerative diseases. Additionally, we examine existing putative therapeutic strategies to efficiently remove cytotoxic proteins from degenerating neurons.


Assuntos
Animais , Humanos , Doença de Alzheimer/tratamento farmacológico , Peptídeos beta-Amiloides/metabolismo , Esclerose Lateral Amiotrófica/tratamento farmacológico , Autofagia/efeitos dos fármacos , Proteínas de Ligação a DNA/metabolismo , Doença de Huntington/tratamento farmacológico , Lisossomos/metabolismo , Terapia de Alvo Molecular , Mutação , Proteínas do Tecido Nervoso/genética , Doenças Neurodegenerativas/tratamento farmacológico , Doença de Parkinson/tratamento farmacológico , Proteínas PrPSc/metabolismo , Doenças Priônicas/tratamento farmacológico , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise , Deficiências na Proteostase/metabolismo , Superóxido Dismutase/metabolismo , Ubiquitina/metabolismo , alfa-Sinucleína/metabolismo , Proteínas tau/metabolismo
2.
Journal of Korean Medical Science ; : 1604-1609, 2014.
Artigo em Inglês | WPRIM | ID: wpr-110673

RESUMO

Misfolded isoform of prion protein (PrP), termed scrapie PrP (PrP(Sc)), tends to aggregate into various fibril forms. Previously, we reported various conditions that affect aggregation of recombinant PrP into amyloids. Because amyloidogenesis of PrP is closely associated with transmissible spongiform encephalopathies such as Creutzfeldt-Jakob disease in humans, we investigated infectivity of recombinant PrP amyloids generated in vitro. Using cultured cell lines which overexpress cellular PrP of different species, we measured the level of de novo synthesized PrP(Sc) in cells inoculated with recombinant mouse PrP amyloids. While PrP-overexpressing cells were susceptible to mouse-adapted scrapie prions used as the positive control, demonstrating the species barrier effect, infection with amyloids made of truncated recombinant PrP (PrP[89-230]) failed to form and propagate PrP(Sc) even in the cells that express mouse cellular PrP. This suggests that infectivity of PrP amyloids generated in vitro is different from that of natural prions. Recombinant PrP (89-230) amyloids tested in the current study retain no or a minute level, if any, of prion infectivity.


Assuntos
Animais , Camundongos , Coelhos , Linhagem Celular , Rim/metabolismo , Proteínas PrPSc/metabolismo , Doenças Priônicas/metabolismo , Príons/metabolismo , Proteínas Recombinantes/metabolismo , Regulação para Cima
3.
Braz. j. med. biol. res ; 38(8): 1223-1231, Aug. 2005. ilus, tab
Artigo em Inglês | LILACS | ID: lil-405524

RESUMO

Crude brain homogenates of terminally diseased hamsters infected with the 263 K strain of scrapie (PrP Sc) were heated and/or pressurized at 800 MPa at 60°C for different times (a few seconds or 5, 30, 120 min) in phosphate-buffered saline (PBS) of different pH and concentration. Prion proteins were analyzed on immunoblots for their proteinase K (PK) resistance, and in hamster bioassays for their infectivity. Samples pressurized under initially neutral conditions and containing native PrP Sc were negative on immunoblots after PK treatment, and a 6-7 log reduction of infectious units per gram was found when the samples were pressurized in PBS of pH 7.4 for 2 h. A pressure-induced change in the protein conformation of native PrP Sc may lead to less PK resistant and less infectious prions. However, opposite results were obtained after pressurizing native infectious prions at slightly acidic pH and in PBS of higher concentration. In this case an extensive fraction of native PrP Sc remained PK resistant after pressure treatment, indicating a protective effect possibly due to induced aggregation of prion proteins in such buffers.


Assuntos
Animais , Cricetinae , Endopeptidase K/química , Pressão Hidrostática , Proteínas PrPSc/química , Soluções Tampão , Encéfalo/metabolismo , Físico-Química , Endopeptidase K/metabolismo , Concentração de Íons de Hidrogênio , Proteínas PrPSc/metabolismo , Proteínas PrPSc/patogenicidade , Fatores de Tempo
4.
Rev. argent. transfus ; 28(1/2): 69-80, ene.-jun. 2002.
Artigo em Espanhol | LILACS | ID: lil-337486

RESUMO

El objetivo de esta reseña es actualizar información ya publicada sobre los priones y las patologías que éstos transmiten. La existencia de una nueva variante de la enfermedad de Creutzfeld-Jakob y la confirmación experimental de que es causada por la misma cepa de priones que la encefalopatía espongiforme bovina (BSE), ha incrementado dramáticamente la necesidad de una precisa comprensión de las bases moleculares de la propagación priónica. El agente infeccioso es una proteína cuya conformación se encuentra alterada, que se reproduce a sí misma convirtiendo una proteína normal en una proteína con conformación priónica. La observación de que los priones se replican en los órganos linfoides en estadios muy tempranos de la infección lleva a cuestionar sobre cuáles son los requerimientos de tipo celular a ser infectado en el sistema linforreticular. Las células dendríticas foliculares serían el sitio de elección para la replicación y el reservorio de priores. El diagnóstico de las enfermedades producidas por priones presenta una serie de problemas debido a las peculiaridades de este tipo de patologías. Considerando que los priones se replican en el sistema linforreticular y posteriormente migran al sistema nervioso central, existe un lapso durante el cual podría propagarse este agente infeccioso por medio de la sangre, sus componentes o sus derivados. De esta forma representaría una nueva patología con la potencial capacidad de transmisión transfusional. Esta nueva forma de herencia independiente de los ácidos nucleicos obliga a replantear el axioma de transferencia de la información genética, hasta el momento, y con concordancia con la teoría evolutiva de Darwin, sólo pensando mediante moléculas constituidas por nucleótidos. ¿Será tiempo de cambiar el paradigma?


Assuntos
Humanos , Animais , Bovinos , Camundongos , Doadores de Sangue , Transfusão de Sangue , Encefalopatia Espongiforme Bovina , Doenças Priônicas/epidemiologia , Doenças Priônicas/etiologia , Doenças Priônicas/fisiopatologia , Doenças Priônicas/prevenção & controle , Doenças Priônicas/transmissão , Biologia Molecular , Príons , Proteínas PrPSc/farmacocinética , Proteínas PrPSc/metabolismo , Síndrome de Creutzfeldt-Jakob/diagnóstico , Síndrome de Creutzfeldt-Jakob/epidemiologia , Síndrome de Creutzfeldt-Jakob/etiologia , Síndrome de Creutzfeldt-Jakob/fisiopatologia , Síndrome de Creutzfeldt-Jakob/história , Síndrome de Creutzfeldt-Jakob/transmissão , Células Dendríticas Foliculares/patologia , Infecções do Sistema Nervoso Central , Controle de Infecções , Suínos
5.
Braz. j. med. biol. res ; 32(7): 853-9, July 1999.
Artigo em Inglês | LILACS | ID: lil-234891

RESUMO

Prions are an unconventional form of infectious agents composed only of protein and involved in transmissible spongiform encephalopathies in humans and animals. The infectious particle is composed by PrPsc which is an isoform of a normal cellular glycosyl-phosphatidylinositol (GPI) anchored protein, PrPc, of unknown function. The two proteins differ only in conformation, PrPc is composed of 40 percent helix while PrPsc has 60 percent ß-sheet and 20 percent helix structure. The infection mechanism is trigged by interaction of PrPsc with cellular prion protein causing conversion of the latter's conformation. Therefore, the infection spreads because new PrPsc molecules are generated exponentially from the normal PrPc. The accumulation of insoluble PrPsc is probably one of the events that lead to neuronal death. Conflicting data in the literature showed that PrPc internalization is mediated either by clathrin-coated pits or by caveolae-like membranous domains. However, both pathways seem to require a third protein (a receptor or a prion-binding protein) either to make the connection between the GPI-anchored molecule to clathrin or to convert PrPc into PrPsc. We have recently characterized a 66-kDa membrane receptor which binds PrPc in vitro and in vivo and mediates the neurotoxicity of a human prion peptide. Therefore, the receptor should have a role in the pathogenesis of prion-related diseases and in the normal cellular process. Further work is necessary to clarify the events triggered by the association of PrPc/PrPsc with the receptor


Assuntos
Camundongos , Humanos , Animais , Cricetinae , Doenças Priônicas/metabolismo , Receptores de Superfície Celular/metabolismo , Proteínas PrPC/análise , Proteínas PrPC/metabolismo , Proteínas PrPSc/análise , Proteínas PrPSc/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA