RESUMO
OBJECTIVE@#To determine and verify the correlation formula of age estimation using the content of signal joint T-cell receptor excision DNA circle (sjTREC) in human peripheral blood and to discuss its application value in forensic biological practice.@*METHODS@#The samples of peripheral blood stains were collected from 30 healthy unrelated individuals whose ages were known. The DNAs were extracted from the samples stored at room temperature after 4 weeks. The content of sjTREC was measured by real-time fluorescent quantitative PCR technique, and the TATA box binding protein (TBP) was selected as reference genes. The age of each sample was predicted with the formula which was Age = -7.181 5 Y-42.458 +/- 9.42 (Y = dCtTBP-sjTREC), and the result was compared with the real age of each individual to determine the accuracy of the formula.@*RESULTS@#sjTREC and TBP gene were detectable in all 30 samples of peripheral blood. The contents of sjTREC in human peripheral blood showed a decreasing tendency with aging. The accuracy rate for the age estimation by this method was 76.67%.@*CONCLUSION@#The method for the age estimation with the content of sjTREC was simple, fast, sensitive, and good species specific with important potential application prospect.
Assuntos
Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Envelhecimento/sangue , Manchas de Sangue , DNA/genética , Primers do DNA/genética , Genética Forense/métodos , Rearranjo Gênico do Linfócito T/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Sensibilidade e Especificidade , Proteína de Ligação a TATA-Box/genéticaRESUMO
En este artículo describimos la distribución anatómica, las características histológicas y moleculares de 32 casos de LNH. La estadificación clínica y clasificación histológica por grados se hizo de acuerdo a esquemas aceptados convencionalmente. Los arreglos detectados en genes que codifican para Ig o el RcT sirvieron para identificar la estirpe celular y el estadio de diferenciación de las células neoplásicas. El análisis de 26 muestras de suero reveló la existencia de anticuerpos contra epítopes de EBV; ocho de estos pacientes contenían secuencias virales integradas en el genoma del tumor. Nuestros estudios indican que el uso de diferentes métodos es fundamental para profundizar en el conocimiento de la historia natural de los LNH.