Characterization and Short-Term clinical study of clay facial mask / Caracterização e estudo clínico de curta duração de máscara facial argilosa

Clay mineral facial masks are used to treat some dermatological diseases, just for cleansing or reduce the amount of oil secreted by sebaceous glands. There are several types of clays, which vary in mineralogical and chemical composition, color and origin. However, the literature lacks studies involving clay facial masks, in particular regarding their influence on skin´s biomechanical properties. Thus, this work aimed to characterize colored clays and evaluate its influence on skin frmness and elasticity by a short-term clinical study. Different clays (named in this study magnesium aluminum silicate - MAS, white, pink and green) were chemically characterized, and facial mask formulations were prepared. The short-term clinical study was performed through the application of formulations on the skin. The skin frmness and elasticity were assessed before treatment and after mask removal. The statistical analysis showed no signifcant influence of time or formulations in those parameters, although volunteers reported the sensation of mechanical tension after the removal of the clay facial masks. Thus, the composition of the different clays did not affect the skin viscoelasticity behavior in the short-term clinical study, and a long-term use of this type of formulation must be indicated to observe all the expected benefts.(AU)

Máscaras faciais argilosas são utilizadas para tratar algumas doenças dermatológicas, apenas para a limpeza ou reduzir a quantidade de óleo secretado pelas glândulas sebáceas. Existem vários tipos de argilas, que variam em composição mineral, química, cor e origem. No entanto, a literatura carece de estudos envolvendo máscaras faciais argilosas, em particular em relação a sua influência nas propriedades biomecânicas da pele. Assim, este trabalho teve como objetivo caracterizar argilas coloridas e avaliar sua influência sobre a frmeza e elasticidade da pele por meio de um estudo clínico de curto prazo. Diferentes argilas (chamadas neste estudo de silicato de alumínio e magnésio - MAS, branca, rosa e verde) foram caracterizadas quimicamente, e formulações de máscaras faciais foram preparadas. O estudo clínico de curto prazo foi realizado por meio da aplicação das formulações na pele. A frmeza e elasticidade da pele foram avaliadas antes do tratamento e após a remoção da máscara. A análise estatística mostrou nenhuma influência signifcativa do tempo ou das formulações nesses parâmetros, embora os voluntários tenham reportado sensação de tensão mecânica, após a remoção das máscaras faciais argilosas. Assim, a composição das diferentes argilas não afetou o comportamento visco-elástico da pele no estudo clínico de curto prazo, e uma utilização de longa duração poderia ser indicada com a fnalidade de se observar todos os benefícios esperados.(AU)

Measurement uncertainty assessment of magnesium trisilicate column for determination of Sudan colorants in food by HPLC using C8 column / 华中科技大学学报（医学）（英德文版）

This study aimed to conduct measurement uncertainty assessment of a new method for determination of Sudan colorants (Sudan I, II, III and IV) in food by high performance liquid chromatography (HPLC). Samples were extracted with organic solvents (hexane, 20% acetone) and first purified by magnesium trisilicate (2MgO·3SiO2). The Sudan colorants (Sudan I-IV) were also initially separated on C8 by gradient elution using acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases and detected with diode-array detector (DAD). The uncertainty of mathematical model of Sudan I, II, III and IV is based on EURACHEM guidelines. The sources and components of uncertainty were calculated. The experiment gave a good linear relationship over the concentration from 0.4 to 4.0 μg/mL and spiked recoveries were from 74.0% to 97.5%. The limits of determination (LOD) were 48, 61, 36, 58 μg/kg for the four analytes, respectively. The total uncertainty of Sudan colorants (Sudan I, II, III and IV) was 810±30.8, 790±28.4, 750±27.0, 730±50.0 μg/kg, respectively. The recovery uncertainty was the most significant factor contributing to the total uncertainty. The developed method is simple, rapid, and highly sensitive. It can be used for the determination of trace Sudan dyes in food samples. The sources of uncertainty have been identified and uncertainty components have been simplified and considered.

Mechanism of DNA transformation based on mineral nanofibers and method improvement / 生物工程学报

Sepiolite--an inexpensive, resourceful, fibrous yet inoffensive mineral--made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4 degrees C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.

Kinetic properties and storage stability of catalase immobilized on to florisil.

The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10 degrees C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g(-1) and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 x 10(5) and 2.0 x 10(4) micromol H2O2 min(-1) mg protein(-1), respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4 degrees C and 25 degrees C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.

Cephradine antacids interaction studies

The present work comprises of interaction studies of cephradine with antacids. Cephradine is included among the first generation cephalosporin, which is active against a wide range of Gram positive and Gram-negative bacteria including penicillinase-producing staphylococci. Since the presence of complexing ligand may affect the bioavailability of a drug in blood or tissue, therefore, in order to study the probable interaction of cephradine with antacids all the reaction conditions were simulated to natural environments. Antacids are commonly used in patients complaining of GI irritations. The behavior of cephradine in presence of seven antacids i.e., simethicone, magaldrate, magnesium carbonate, magnesium hydroxide, magnesium trisilicate, sodium bicarbonate and aluminium hydroxide was studied by using standard dissolution apparatus. Cephradine was monitored both by UV and by high performance liquid chromatography. The results revealed that antacids containing polyvalent cations retarded the in vitro availability of cephradine. Moreover, these studies indicated that cephradine was strongly adsorbed on antacids; magnesium trisilicate and simeco tablets [powdered] exhibited relatively higher adsorption capacities

Interação enfermeira-cliente e utilização de papaína associada ao silicato de magnésio: uma tecnologia de cuidado de feridas / Nursing-patient interaction and papain's utilization associated with magnesium silicate: a wounds care technology

A papaína (Carica papaya) vem sendo empregada na forma de solução aquosa e salina no tratamento de feridas de várias etiologias, para acelerar o processo de cicatrização. Considerando esta técnica trabalhosa, comecei a utilizar a papaína associada ao silicato de magnésio, obtendo resultados satisfatórios, mas sem ter fundamentado em achados de pesquisa. Motivo pelo qual desenvolvi este estudo com 20 clientes, 8 homens e 12 mulheres, que estavam predominantemente (85%) na faixa etária dos 50 aos 80 anos e foram por mim atendidos em ambulatório do Hospital Universitário Pedro Ernesto da Universidade do Estado do Rio de Janeiro, no período de abril de 2003 a janeiro de 2004.0 estudo, de natureza quantitativa, do tipo exploratório descritivo (Wood & Haber, 2001), teve como objetivo criar e avaliar a eficácia da tecnologia do cuidado que alia interação enfermeira-cliente e a utilização da papaína associada ao silicato de magnésio na maximização da cicatrização de feridas. O referencial do estudo é fundamentado nos conceitos de interação (KING,1981), tecnologia de cuidado (NIETSCHE; LEOPARDI, 2000), procedimento de curativo (ROGENSKI, 1996; MONETA, 1998; ALMEIDA,2000). A amostra foi não probabilística acidental; os sujeitos que atendiam aos critérios assinavam o consentimento livre e esclarecido conforme resoluções 196 e 251 do CNS e eram acompanhados pela pesquisadora, semanalmente e conforme a evolução quinzenalmente, e tomada das medidas lineares da ferida. Foram analisados por meio de estatística descritiva. Os resultados segundo o índice de aproveitamento, utilizando como critério o percentual de comparecimento às consultas agendadas e realização do curativo em domicílio e no consultório, permitiu destacar dois grupos, o A (9 sujeitos, aproveitamento parcial, 74,7% de presença nas consultas previamente agendadas e 95,3% de realização dos curativos) e o B (11 sujeitos, 100%). Este resultado demonstra a eficácia da tecnologia de cuidado no que se refere à intera

Talcose pulmonar associada ao uso endovenoso de medicamentos orais: relato de caso / Pulmonary talcosis associated with intravenous use of oral medications: a case report

Neste trabalho são apresentados os achados observados na tomografia computadorizada de alta resolução de um paciente com talcose pulmonar, com a doença adquirida pelo uso de drogas orais injetadas por via venosa. O principal aspecto observado na tomografia computadorizada de alta resolução foi o de nódulos centrolobulares, associados a massas conglomeradas. Havia também enfisema e áreas de atenuação em vidro fosco. Estes achados são bastante sugestivos de talcose pulmonar

We report the findings of the high-resolution computed tomography of the chest of a patient with pulmonary talcosis related to intravenous injection of diluted oral medicine. The most important high-resolution computed tomography findings were small centrilobular nodules associated with conglomerated masses. Areas of emphysema and ground glass attenuation were also seen. These high-resolution computed tomography findings are highly suggestive of pulmonary talcosis.