Detección y tipificación de virus papiloma humano en lesiones preneoplásicas del cuello uterino mediante PCR-RFLP / Detection and typification of human papilloma virus in pre cancerous cervical lesions

Background: The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions. Aim: To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. Material and methods: A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism. Results: All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76 percent of women. Fourteen percent had an infection with multiple subtypes and in 10 percent, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53. Conclusions: There is a high rate of infection with HPV with a high oncogenic risk among these women.

Detection of HPV DNA in cervical carcinomas by PCR and hybrid capture assay.

HPV DNA was detected in exfoliated cervical cells of 73% (85/116) cervical cancer patients by PCR using HPV consensus primers and by hybrid capture assay (HC II) (Digene Corp., USA) in 77 of the 85 cases found HPV positive by PCR. Presence of HPV 16/18 DNA were investigated in the 79 cases by PCR using type specific primers. HPV 16 was detected in 31 (39%) patients, HPV 18 in 7 (8.8%), both HPV 16 and 18 in 19 (24%) and HPVs other than 16/18 in 22 (27.8%) cases. Age and clinical stages had no significant effect on HPV prevalence. Double infection of HPV 16 and 18 was significantly (p<0.05) high in the older patients (56 years or more) compared to younger group. Results indicated that cervical cancers in India are strongly associated with high-risk type HPV infection. HC II assays and PCR results for detection of HPV in cervical smears were comparable.

Hallazgos de secuencias genómicas de H.P.V. en células mononucleres de sangre periférica / Discoveries of HPV genomic sequences in mononuclear cells of it outlying blood

El factor inmunológico además del tipo viral, ha sido involucreado en la cronicidad y evolución de las lesiones del TGI por HPV. En este trabajo hemos investigado la presencia de secuencias de DNA de HPV en células mononucleares de sangre periférica (CMSP) de mujeres sanas y con lesiones de TGI astribuídas a infecciones por HPV

Determinación inmunohistoquímica de la proteína p53 en biopsias de cuello uterino / Immunohistochemistry determination of the p53 protein in cervical biopsy

El gen p53 regula el ingreso de las células a la fase S del ciclo celular mediante la síntesis de una proteína efectora. La mutación de este gen se relacionaría con la pérdida de la capacidad supresora y la concomitante progresión tumoral. La génesis del carcinoma de cérvix podría vincularse a la mutación del gen p53 por acción del ADN viral (HPV). Realizamos la inmunomarcación de la proteína p53 en 88 biopsias de cérvix. De éstas, 10 correspondieron a cuello normal, 41 a lesiones intraepiteliales de bajo grado, 24 a lesiones intraepiteliales de alto grado y 13 a carcinomas invasores. La positividad fue del 10 por ciento, 58,5 por ciento, 70,8 por ciento y 92,3 por ciento respectivamente. Se evaluó también: atipia coilocitótica, reacción inflamatoria, localización e intensidad de la inmunomarcación, tipo de carcinoma invasor, etc. En este informe queda demostrado que el mayor grado de lesión correlaciona con mayor expresión de proteína p53 anómala o mutada