Assuntos
Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Vírus da Febre Aftosa/isolamento & purificação , Febre Aftosa/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Doença Aguda , Líquidos Corporais/virologia , Doenças dos Bovinos/virologia , Esôfago/virologia , Vírus da Febre Aftosa/classificação , Vírus da Febre Aftosa/genética , Vírus da Febre Aftosa/imunologia , Febre Aftosa/virologia , Faringe/virologia , Reprodutibilidade dos Testes , RNA Viral/análise , Reação em Cadeia da Polimerase em Tempo Real/instrumentação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/instrumentação , Sensibilidade e Especificidade , Manejo de Espécimes , Vacinação/veterinária , Vacinas Virais/imunologiaRESUMO
Foot and mouth disease [FMD] is a highly contagious viral disease of ruminant which causes fever and postule on mouth, hoof and teat. The main purpose of this study was to determine and charachtrize isolated FMD virus from Iran between 2005-2006, and to compare it with vaccine virus strains. After preparation of samples, serological test for typing of virus was performed. In order to virus isolation. the samples were inoculated to IBRS2 cell, RT-PCR and PCR were used for sequencing. Two dimentional virus neutralization test was carried out for detecting of immunological relationship [r value] between the field isolate and virus presented in vaccine. Detected strains were as follows: 241 samples of type AO5IR, 125 of type A87IR, 79 of type 0.3 of type Asia and 714 negative out of 1162 samples. Average r-values of type A, 0, Asia field virus with vaccine strains were 50-92% and 97%, respectively. Phylogenic tree was designed according to the nucleic acid sequencing data. There is not strong relationship between field viruses of type A and vaccine viruses. However a strong relationship was shown for type 0 and Asia ones with vaccine virus strains