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Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods.
Emmerich, Petra; von Possel, Ronald; Hemmer, Christoph Josef; Fritzsche, Carlos; Geerdes-Fenge, Hilte; Menge, Babett; Messing, Claudia; Borchardt-Lohölter, Viola; Deschermeier, Christina; Steinhagen, Katja.
  • Emmerich P; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Center for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany.
  • von Possel R; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany.
  • Hemmer CJ; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Center for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany.
  • Fritzsche C; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany.
  • Geerdes-Fenge H; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany.
  • Menge B; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany.
  • Messing C; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany.
  • Borchardt-Lohölter V; Institute for Experimental Immunology, Affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany.
  • Deschermeier C; Institute for Experimental Immunology, Affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany.
  • Steinhagen K; Institute for Experimental Immunology, Affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany.
J Med Virol ; 93(10): 5816-5824, 2021 10.
Article in English | MEDLINE | ID: covidwho-1453607
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Semantic information from SemMedBD (by NLM)
1. Anti-Antibodies PART_OF 2019 novel coronavirus
Subject
Anti-Antibodies
Predicate
PART_OF
Object
2019 novel coronavirus
2. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
3. Assay USES CD40LG wt Allele|CD40LG
Subject
Assay
Predicate
USES
Object
CD40LG wt Allele|CD40LG
4. Antigens PART_OF 2019 novel coronavirus
Subject
Antigens
Predicate
PART_OF
Object
2019 novel coronavirus
5. Anti-Antibodies PART_OF 2019 novel coronavirus
Subject
Anti-Antibodies
Predicate
PART_OF
Object
2019 novel coronavirus
6. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
7. Assay USES CD40LG wt Allele|CD40LG
Subject
Assay
Predicate
USES
Object
CD40LG wt Allele|CD40LG
8. Antigens PART_OF 2019 novel coronavirus
Subject
Antigens
Predicate
PART_OF
Object
2019 novel coronavirus
ABSTRACT
Serological testing for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction-confirmed coronavirus disease 2019 (COVID-19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme-linked immunosorbent assays. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information on whether or not the individual has been infected with SARS-CoV-2. Enzyme-linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Observational study / Risk factors Language: English Journal: J Med Virol Clinical aspect: Diagnosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Observational study / Risk factors Language: English Journal: J Med Virol Clinical aspect: Diagnosis Year: 2021
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