The kinetics of viral load and antibodies to SARS-CoV-2.
Clin Microbiol Infect
; 26(12): 1690.e1-1690.e4, 2020 Dec.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1018998
ABSTRACT
OBJECTIVES:
The aim was to understand persistence of the virus in body fluids the and immune response of an infected host to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), an agent of coronavirus disease 2019 (COVID-19).METHODS:
We determined the kinetics of viral load in several body fluids through real time reverse transcription polymerase chain reaction, serum antibodies of IgA, IgG and IgM by enzyme-linked immunosorbent assay and neutralizing antibodies by microneutralization assay in 35 COVID-19 cases from two hospitals in Guangdong, China.RESULTS:
We found higher viral loads and prolonged shedding of virus RNA in severe cases of COVID-19 in nasopharyngeal (1.3 × 106 vs 6.4 × 104, p < 0.05; 7â¼8 weeks) and throat (6.9 × 106 vs 2.9 × 105, p < 0.05; 4â¼5 weeks), but similar in sputum samples (5.5 × 106 vs 0.9 × 106, p < 0.05; 4â¼5 weeks). Viraemia was rarely detected (2.8%, n = 1/35). We detected early seroconversion of IgA and IgG at the first week after illness onset (day 5, 5.7%, n = 2/35). Neutralizing antibodies were produced in the second week, and observed in all 35 included cases after the third week illness onset. The levels of neutralizing antibodies correlated with IgG (rs = 0.85, p < 0.05; kappa = 0.85) and IgA (rs = 0.64, p < 0.05; kappa = 0.61) in severe, but not mild cases (IgG, rs = 0.42, kappa = 0.33; IgA, rs = 0.32, kappa = 0.22). No correlation with IgM in either severe (rs = 0.17, kappa = 0.06) or mild cases (rs = 0.27, kappa = 0.15) was found.DISCUSSION:
We revealed a prolonged shedding of virus RNA in the upper respiratory tract, and evaluated the consistency of production of IgG, IgA, IgM and neutralizing antibodies in COVID-19 cases.Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Líquidos Corporales
/
Esparcimiento de Virus
/
Carga Viral
/
COVID-19
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio experimental
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Clin Microbiol Infect
Asunto de la revista:
Enfermedades Transmisibles
/
Microbiologia
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
J.cmi.2020.08.043
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