The m6A methylome of SARS-CoV-2 in host cells.
Cell Res
; 31(4): 404-414, 2021 04.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1054016
ABSTRACT
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. The molecular mechanism of interaction between host and viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 genomic RNA, as well as the negative-sense RNA, is dynamically N6-methyladenosine (m6A)-modified in human and monkey cells. Combined RIP-seq and miCLIP analyses identified a total of 8 m6A sites at single-base resolution in the genome. Especially, epidemic strains with mutations at these identified m6A sites have emerged worldwide, and formed a unique cluster in the US as indicated by phylogenetic analysis. Further functional experiments showed that m6A methylation negatively regulates SARS-CoV-2 infection. SARS-CoV-2 infection also triggered a global increase in host m6A methylome, exhibiting altered localization and motifs of m6A methylation in mRNAs. Altogether, our results identify m6A as a dynamic epitranscriptomic mark mediating the virus-host interaction.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Adenosina
/
Genoma Viral
/
SARS-CoV-2
Tipo de estudio:
Ensayo controlado aleatorizado
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Cell Res
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
S41422-020-00465-7
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