Your browser doesn't support javascript.
Performance Characteristics of Severe Acute Respiratory Syndrome Coronavirus 2 RT-PCR Tests in a Single Health System: Analysis of >10,000 Results from Three Different Assays.
Price, Travis K; Bowland, Brian C; Chandrasekaran, Sukantha; Garner, Omai B; Yang, Shangxin.
  • Price TK; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California.
  • Bowland BC; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California.
  • Chandrasekaran S; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California.
  • Garner OB; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California.
  • Yang S; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California. Electronic address: shangxinyang@mednet.ucla.edu.
J Mol Diagn ; 23(2): 159-163, 2021 02.
Artículo en Inglés | MEDLINE | ID: covidwho-1065363
ABSTRACT
The current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in the approval of numerous molecular diagnostic assays with various performance and technical capacities. There are limited data comparing performance among assays. We conducted a retrospective analysis of >10,000 test results among three widely used RT-PCR assays for coronavirus disease 2019 (CDC, Simplexa Direct, and TaqPath) to assess performance characteristics. We also retested remnant weakly positive specimens to assess analytical sensitivity. All assays had strong linear correlation and little bias among CT values for PCR targets. In patients with first-test negative results (n = 811), most (795, 98.0%) remained negative for all subsequent testing. Retesting of weakly positive specimens (CT > 30) showed sensitivities as follows TaqPath (97.8%), CDC (91%), Simplexa (75.3%). Our analysis showed no performance difference among PCR targets within the same assay, suggesting a single target is sufficient for SARS-CoV-2 detection. Lower respiratory tract specimens had a higher negative predictive value (100%) than upper respiratory tract specimens (98%), highlighting the utility of testing lower respiratory tract specimens when clinically indicated. Negative predictive value did not increase on further repeated testing, providing strong evidence for discouraging unnecessary repeated testing for SARS-CoV-2.
Asunto(s)

Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Bioensayo / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Prueba de COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio observacional / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: J Mol Diagn Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Bioensayo / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Prueba de COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio observacional / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: J Mol Diagn Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo