CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes.
Genome Med
; 13(1): 21, 2021 02 09.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1076156
ABSTRACT
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, ≥ 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Genoma Viral
/
Pandemias
/
SARS-CoV-2
/
COVID-19
Límite:
Humanos
Idioma:
Inglés
Revista:
Genome Med
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
S13073-021-00839-5
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