Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.
Talanta
; 227: 122154, 2021 May 15.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1078200
ABSTRACT
Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
ADN Viral
/
ARN Viral
/
Pyrococcus furiosus
/
Reacción en Cadena de la Ligasa
/
Proteínas Argonautas
Tipo de estudio:
Estudios diagnósticos
Tópicos:
Covid persistente
Límite:
Humanos
Idioma:
Inglés
Revista:
Talanta
Año:
2021
Tipo del documento:
Artículo
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