SARS-CoV-2 in hospital indoor environments is predominantly non-infectious.
Virol J
; 18(1): 109, 2021 06 02.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1388777
ABSTRACT
BACKGROUND:
The ongoing SARS-CoV-2 pandemic has spread rapidly worldwide and disease prevention is more important than ever. In the absence of a vaccine, knowledge of the transmission routes and risk areas of infection remain the most important existing tools to prevent further spread.METHODS:
Here we investigated the presence of the SARS-CoV-2 virus in the hospital environment at the Uppsala University Hospital Infectious Disease ward by RT-qPCR and determined the infectivity of the detected virus in vitro on Vero E6 cells.RESULTS:
SARS-CoV-2 RNA was detected in several areas, although attempts to infect Vero E6 cells with positive samples were unsuccessful. However, RNase A treatment of positive samples prior to RNA extraction did not degrade viral RNA, indicating the presence of SARS-CoV-2 nucleocapsids or complete virus particles protecting the RNA as opposed to free viral RNA.CONCLUSION:
Our results show that even in places where a moderate concentration (Ct values between 30 and 38) of SARS-CoV-2 RNA was found; no infectious virus could be detected. This suggests that the SARS-CoV-2 virus in the hospital environment subsides in two states; as infectious and as non-infectious. Future work should investigate the reasons for the non-infectivity of SARS-CoV-2 virions.Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Monitoreo del Ambiente
/
Infección Hospitalaria
/
Transmisión de Enfermedad Infecciosa
/
COVID-19
Tipo de estudio:
Estudio observacional
/
Estudio pronóstico
/
Ensayo controlado aleatorizado
Tópicos:
Vacunas
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Virol J
Asunto de la revista:
Virología
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
S12985-021-01556-6
Similares
MEDLINE
...
LILACS
LIS