Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
Mar Drugs
; 19(12)2021 Nov 30.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1542656
ABSTRACT
The COVID-19 pandemic is a major human health concern. The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2). In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor. Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities. In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro. The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance. RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR). In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL). RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin. Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Algas Marinas
/
Desoxiazúcares
/
Extractos Vegetales
/
SARS-CoV-2
/
Tratamiento Farmacológico de COVID-19
/
Mananos
Tipo de estudio:
Estudio experimental
Tópicos:
Variantes
Límite:
Humanos
Idioma:
Inglés
Asunto de la revista:
Biologia
/
Farmacología
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Md19120685
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