A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.
Proc Natl Acad Sci U S A
; 118(50)2021 12 14.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1559358
ABSTRACT
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created an urgent need for new technologies to treat COVID-19. Here we report a 2'-fluoro protected RNA aptamer that binds with high affinity to the receptor binding domain (RBD) of SARS-CoV-2 spike protein, thereby preventing its interaction with the host receptor ACE2. A trimerized version of the RNA aptamer matching the three RBDs in each spike complex enhances binding affinity down to the low picomolar range. Binding mode and specificity for the aptamer-spike interaction is supported by biolayer interferometry, single-molecule fluorescence microscopy, and flow-induced dispersion analysis in vitro. Cell culture experiments using virus-like particles and live SARS-CoV-2 show that the aptamer and, to a larger extent, the trimeric aptamer can efficiently block viral infection at low concentration. Finally, the aptamer maintains its high binding affinity to spike from other circulating SARS-CoV-2 strains, suggesting that it could find widespread use for the detection and treatment of SARS-CoV-2 and emerging variants.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Aptámeros de Nucleótidos
/
Internalización del Virus
/
SARS-CoV-2
Tipo de estudio:
Estudios diagnósticos
/
Revisión sistemática/Meta análisis
Tópicos:
Variantes
Límite:
Humanos
Idioma:
Inglés
Año:
2021
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS