A DNA Barcode-Based Aptasensor Enables Rapid Testing of Porcine Epidemic Diarrhea Viruses in Swine Saliva Using Electrochemical Readout.
Angew Chem Int Ed Engl
; 61(31): e202204252, 2022 08 01.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1843851
ABSTRACT
Pen-side testing of farm animals for infectious diseases is critical for preventing transmission in herds and providing timely intervention. However, most existing pathogen tests have to be conducted in centralized labs with sample-to-result times of 2-4â
days. Herein we introduce a test that uses a dual-electrode electrochemical chip (DEE-Chip) and a barcode-releasing electroactive aptamer for rapid on-farm detection of porcine epidemic diarrhea viruses (PEDv). The sensor exploits inter-electrode spacing reduction and active field mediated transport to accelerate barcode movement from electroactive aptamers to the detection electrode, thus expediting assay operation. The test yielded a clinically relevant limit-of-detection of 6â
nM (0.37â
µg mL-1 ) in saliva-spiked PEDv samples. Clinical evaluation of this biosensor with 12 porcine saliva samples demonstrated a diagnostic sensitivity of 83 % and specificity of 100 % with a concordance value of 92 % at an analysis time of one hour.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
/
Infecciones por Coronavirus
/
Virus de la Diarrea Epidémica Porcina
Tipo de estudio:
Estudios diagnósticos
/
Estudio experimental
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Angew Chem Int Ed Engl
Año:
2022
Tipo del documento:
Artículo
País de afiliación:
Anie.202204252
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