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Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination.
Kenny, Grace; Negi, Riya; O'Reilly, Sophie; Garcia-Leon, Alejandro; Alalwan, Dana; Gaillard, Colette Marie; Saini, Gurvin; Inzitari, Rosana; Feeney, Eoin R; Yousif, Obada; Cotter, Aoife G; de Barra, Eoghan; Sadlier, Corinna; Crispie, Fiona; Doran, Peter; Gautier, Virginie; Mallon, Patrick W G.
  • Kenny G; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland; Department of Infectious Diseases, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland. Electronic address: grace.kenny1@ucd.ie.
  • Negi R; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • O'Reilly S; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Garcia-Leon A; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Alalwan D; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Gaillard CM; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Saini G; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Inzitari R; School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
  • Feeney ER; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland; Department of Infectious Diseases, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
  • Yousif O; Endocrinology Department, Wexford General Hospital, Carricklawn, Wexford, Ireland.
  • Cotter AG; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland; Department of Infectious Diseases, Mater Misericordiae University Hospital, Eccles St, Dublin 7, Ireland.
  • de Barra E; Department of Infectious Diseases, Beaumont Hospital, Beaumont, Dublin 9, Ireland; Department of International Health and Tropical Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland.
  • Sadlier C; Department of Infectious Diseases, Cork University Hospital, Wilton, Co Cork, Ireland.
  • Crispie F; Teagasc Food Research Centre, Moorepark, Ireland; APC Microbiome Ireland, Cork, Ireland.
  • Doran P; School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
  • Gautier V; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland.
  • Mallon PWG; Centre for Experimental Pathogen Host Research (CEPHR), University College Dublin, Belfield, Dublin 4, Ireland; Department of Infectious Diseases, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
J Immunol Methods ; 510: 113345, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: covidwho-2004232
ABSTRACT
Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8-3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen.
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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio experimental / Estudio pronóstico Tópicos: Vacunas Límite: Humanos Idioma: Inglés Revista: J Immunol Methods Año: 2022 Tipo del documento: Artículo

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio experimental / Estudio pronóstico Tópicos: Vacunas Límite: Humanos Idioma: Inglés Revista: J Immunol Methods Año: 2022 Tipo del documento: Artículo