The immunopathology of COVID-19 placentitis: A multi-omic spatial approach
American Journal of Reproductive Immunology
; 89(Supplement 1):40, 2023.
Artículo
en Inglés
| EMBASE | ID: covidwho-20241541
ABSTRACT
Problem:
COVID-19 placentitis is a rare complication of maternal SARS-CoV-2 respiratory infection associated with serious adverse obstetric outcomes, including intra-uterine death. The precise role of SARS-CoV-2 in COVID-19 placentitis is uncertain, as trophoblast infection is only observed in around one-half of the affected placenta. Method of Study Through multi-omic spatial profiling, including Nanostring GeoMX digital spatial profiling and Lunaphore COMET multiplex IHC, we provide a deep characterization of the immunopathology of placentitis from obstetrically complicated maternal COVID-19 infection. Result(s)We show that SARS-CoV-2 infection of placental trophoblasts is associated with a distinct innate and adaptive immune cell infiltrate, florid cytokine expression and upregulation of viral restriction factors. Quantitative spatial analyses reveal a unique microenvironment surrounding virus-infected trophoblasts characterizedd by multiple immune evasion mechanisms, including immune checkpoint expression, cytotoxic T-cell exclusion, and interferon blunting. Placental viral loads inversely correlated with the duration of maternal infection consistent with progressive virus clearance, potentially explaining the absence of virus in some cases. Conclusion(s) Our results demonstrate a central role for placental SARS-CoV-2 infection in driving the unique immunopathology of COVID-19 placentitis.
cell infiltration; conference abstract; controlled study; coronavirus disease 2019; cytotoxic T lymphocyte; female; gene expression; human; human cell; human tissue; immune evasion; immunocompetent cell; immunopathology; maternal infection; microenvironment; placenta; protein expression; quantitative analysis; spatial analysis; trophoblast; upregulation; viral clearance; virus load; cytokine; endogenous compound; interferon
Texto completo:
Disponible
Colección:
Bases de datos de organismos internacionales
Base de datos:
EMBASE
Idioma:
Inglés
Revista:
American Journal of Reproductive Immunology
Año:
2023
Tipo del documento:
Artículo
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