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Evaluation of SARS-CoV-2 isolation in cell culture from nasal/nasopharyngeal swabs or saliva specimens of patients with COVID-19.
Yazawa, Shunsuke; Yamazaki, Emiko; Saga, Yumiko; Itamochi, Masae; Inasaki, Noriko; Shimada, Takahisa; Oishi, Kazunori; Tani, Hideki.
  • Yazawa S; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Yamazaki E; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Saga Y; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Itamochi M; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Inasaki N; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Shimada T; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan.
  • Oishi K; Toyama Institute of Health, Toyama, Japan.
  • Tani H; Department of Virology, Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama, 939-0363, Japan. toyamaeiken3@juno.ocn.ne.jp.
Sci Rep ; 13(1): 8893, 2023 06 01.
Artículo en Inglés | MEDLINE | ID: covidwho-20245029
ABSTRACT
It has been revealed that SARS-CoV-2 can be efficiently isolated from clinical specimens such as nasal/nasopharyngeal swabs or saliva in cultured cells. In this study, we examined the efficiency of viral isolation including SARS-CoV-2 mutant strains between nasal/nasopharyngeal swab or saliva specimens. Furthermore, we also examined the comparison of viral isolation rates by sample species using simulated specimens for COVID-19. As a result, it was found that the isolation efficiency of SARS-CoV-2 in the saliva specimens was significantly lower than that in the nasal/nasopharyngeal swab specimens. In order to determine which component of saliva is responsible for the lower isolation rate of saliva specimens, we tested the abilities of lactoferrin, amylase, cathelicidin, and mucin, which are considered to be abundant in saliva, to inhibit the infection of SARS-CoV-2 pseudotyped viruses (SARS-CoV-2pv). Lactoferrin and amylase were found to inhibit SARS-CoV-2pv infection. In conclusion, even if the same number of viral genome copies was detected by the real-time RT-PCR test, infection of SARS-CoV-2 present in saliva is thought to be inhibited by inhibitory factors such as lactoferrin and amylase, compared to nasal/nasopharyngeal swab specimens.
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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio experimental / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Sci Rep Año: 2023 Tipo del documento: Artículo País de afiliación: S41598-023-35915-w

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio experimental / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Sci Rep Año: 2023 Tipo del documento: Artículo País de afiliación: S41598-023-35915-w