INTEGRATION OF MINIVALVES WITH RNA AMPLIFICATION DEVICE FOR SIMULTANEOUS DETECTION OF SARS-COV-2 AND INFLUENZA VIRUSES
ASME 2022 International Mechanical Engineering Congress and Exposition, IMECE 2022
; 4, 2022.
Artículo
en Inglés
| Scopus | ID: covidwho-2249068
ABSTRACT
We report a point-of-care (POC) device for simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A viruses. The device carries out sample preparation using ball-based valves for sequential delivery of reagents. A microfluidic paper-based analytical device (µPAD) in the detection unit enables RNA isolation and enrichment, followed by reverse transcription loop-mediated isothermal amplification (RT-LAMP) and colorimetric detection. The device integrates all the necessary steps for the sample preparation, including virus lysis, RNA enrichment and purification of two virus samples. The device enabled simultaneous detection of SARS-CoV-2 and Influenza A N1H1 viruses in 50 min., with limit of detection of 2 and 6 genome equivalents (GEs), respectively. The device was also capable of detecting environmental sample of the two viruses. Copyright © 2022 by ASME.
Texto completo:
Disponible
Colección:
Bases de datos de organismos internacionales
Base de datos:
Scopus
Idioma:
Inglés
Revista:
ASME 2022 International Mechanical Engineering Congress and Exposition, IMECE 2022
Año:
2022
Tipo del documento:
Artículo
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