Rapid PCR kit: lateral flow paper strip with Joule heater for SARS-CoV-2 detection.
Mater Horiz
; 10(5): 1697-1704, 2023 05 09.
Artículo
en Inglés
| MEDLINE | ID: covidwho-2266462
ABSTRACT
Polymerase chain reaction (PCR)-based diagnostic kits for point-of-care (POC) testing are highly desirable to prevent the spread of infectious diseases. Here, we demonstrate a rapid PCR testing kit that involves integrating a lateral flow paper strip with a nichrome-based thin film heater. The use of a paper membrane as a PCR-solution container results in fast thermocycling without a cooler because the membrane can contain the solution with a high specific surface area where Joule heating is applied. After PCR, amplified products are simultaneously detected at the lateral flow paper strip with the naked eye. Severe acute respiratory syndrome ß-coronavirus RNA can be detected within 30 min after PCR solution injection. This work reveals that the paper membrane can act as not only a capillary flow channel but also as a promising platform for fast PCR and detection.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
SARS-CoV-2
/
COVID-19
Tipo de estudio:
Estudios diagnósticos
Límite:
Humanos
Idioma:
Inglés
Revista:
Mater Horiz
Año:
2023
Tipo del documento:
Artículo
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