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Fangchinoline Exerts Anticancer Effects on Colorectal Cancer Cells by Evoking Cell Apoptosis via Endoplasmic Reticulum Stress.
Jiang, Xiuling; Hu, Yougen; Peng, Jianming; Luo, Xue; Su, Landi; Tang, Yuanjie.
  • Jiang X; Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • Hu Y; Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China.
  • Peng J; Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • Luo X; Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • Su L; Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • Tang Y; Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China. tangyuanjie1970@126.com.
Bull Exp Biol Med ; 174(5): 639-646, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: covidwho-2315744
ABSTRACT
We studied the anti-tumor effect of fangchinoline (FAN) against human colorectal cancer cell lines CCL-244 and SW480 and analyzed the mechanism of FAN action. The cell viability and apoptosis were assessed by MTT test and Annexin V-PI staining; caspase-3 activity was measured by Western blotting. The expression of endoplasmic reticulum stress-related proteins was assessed by real-time PCR, Western blotting, and gene transfection. It was found that FAN inhibited cell growth and induced apoptosis in human colorectal cancer cell lines CCL-244 and SW480 in a dose-dependent manner. The caspase-3 inhibitor Ac-DEVD-CHO could reverse the inhibitory effect of FAN. Moreover, FAN significantly increased the expression of endoplasmic reticulum stress-related proteins p-PERK, p-eIF2α, ATF4, and CHOP in CCL-244 and SW480 cells. In addition, endoplasmic reticulum stress inhibitor 4-phenylbutyric acid or CHOP knockdown could prevent FAN-induced apoptosis. Thus, FAN induced apoptosis of human colorectal cancer through activation of endoplasmic reticulum stress.
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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Transducción de Señal Tipo de estudio: Estudio experimental Límite: Humanos Idioma: Inglés Revista: Bull Exp Biol Med Año: 2023 Tipo del documento: Artículo País de afiliación: S10517-023-05761-x

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Transducción de Señal Tipo de estudio: Estudio experimental Límite: Humanos Idioma: Inglés Revista: Bull Exp Biol Med Año: 2023 Tipo del documento: Artículo País de afiliación: S10517-023-05761-x