The Architecture of Inactivated SARS-CoV-2 with Postfusion Spikes Revealed by Cryo-EM and Cryo-ET.
Structure
; 28(11): 1218-1224.e4, 2020 11 03.
Artículo
en Inglés
| MEDLINE | ID: covidwho-872505
ABSTRACT
The ongoing global pandemic of coronavirus disease 2019 (COVID-19) resulted from the outbreak of SARS-CoV-2 in December 2019. Currently, multiple efforts are being made to rapidly develop vaccines and treatments to fight COVID-19. Current vaccine candidates use inactivated SARS-CoV-2 viruses; therefore, it is important to understand the architecture of inactivated SARS-CoV-2. We have genetically and structurally characterized ß-propiolactone-inactivated viruses from a propagated and purified clinical strain of SARS-CoV-2. We observed that the virus particles are roughly spherical or moderately pleiomorphic. Although a small fraction of prefusion spikes are found, most spikes appear nail shaped, thus resembling a postfusion state, where the S1 protein of the spike has disassociated from S2. Cryoelectron tomography and subtomogram averaging of these spikes yielded a density map that closely matches the overall structure of the SARS-CoV postfusion spike and its corresponding glycosylation site. Our findings have major implications for SARS-CoV-2 vaccine design, especially those using inactivated viruses.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Propiolactona
/
Virión
/
Desinfectantes
/
Betacoronavirus
Tipo de estudio:
Estudio pronóstico
Tópicos:
Vacunas
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Structure
Asunto de la revista:
Biologia Molecular
/
Bioquímica
/
Biotecnologia
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
J.str.2020.10.001
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