Your browser doesn't support javascript.
Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion.
Zang, Ruochen; Case, James Brett; Yutuc, Eylan; Ma, Xiucui; Shen, Sheng; Gomez Castro, Maria Florencia; Liu, Zhuoming; Zeng, Qiru; Zhao, Haiyan; Son, Juhee; Rothlauf, Paul W; Kreutzberger, Alex J B; Hou, Gaopeng; Zhang, Hu; Bose, Sayantan; Wang, Xin; Vahey, Michael D; Mani, Kartik; Griffiths, William J; Kirchhausen, Tom; Fremont, Daved H; Guo, Haitao; Diwan, Abhinav; Wang, Yuqin; Diamond, Michael S; Whelan, Sean P J; Ding, Siyuan.
  • Zang R; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Case JB; Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, 266100 Qingdao, China.
  • Yutuc E; Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Ma X; Swansea University Medical School, SA2 8PP Swansea, United Kingdom.
  • Shen S; Center for Cardiovascular Research and Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO 63111.
  • Gomez Castro MF; John Cochran VA Medical Center, St. Louis, MO 63106.
  • Liu Z; Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.
  • Zeng Q; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Zhao H; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Son J; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Rothlauf PW; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Kreutzberger AJB; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Hou G; Program in Molecular Cell Biology, Washington University School of Medicine, St. Louis, MO 63110.
  • Zhang H; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Bose S; Program in Virology, Harvard Medical School, Boston, MA 02115.
  • Wang X; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.
  • Vahey MD; Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
  • Mani K; Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
  • Griffiths WJ; Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.
  • Kirchhausen T; Autonomous Therapeutics, Inc., New York, NY 10013.
  • Fremont DH; Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, 266100 Qingdao, China.
  • Guo H; Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63110.
  • Diwan A; Center for Cardiovascular Research and Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO 63111.
  • Wang Y; John Cochran VA Medical Center, St. Louis, MO 63106.
  • Diamond MS; Swansea University Medical School, SA2 8PP Swansea, United Kingdom.
  • Whelan SPJ; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.
  • Ding S; Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A ; 117(50): 32105-32113, 2020 12 15.
Artículo en Inglés | MEDLINE | ID: covidwho-947594
Preprint
Este artículo de revista científica es probablemente basado en un preprint previamente disponible, por medio del reconocimiento de similitud realizado por una máquina. La confirmación humana aún está pendiente.
Ver preprint
ABSTRACT
Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-stimulated gene that shows broad antiviral activities against a wide range of enveloped viruses. Here, using an IFN-stimulated gene screen against vesicular stomatitis virus (VSV)-SARS-CoV and VSV-SARS-CoV-2 chimeric viruses, we identified CH25H and its enzymatic product 25-hydroxycholesterol (25HC) as potent inhibitors of SARS-CoV-2 replication. Internalized 25HC accumulates in the late endosomes and potentially restricts SARS-CoV-2 spike protein catalyzed membrane fusion via blockade of cholesterol export. Our results highlight one of the possible antiviral mechanisms of 25HC and provide the molecular basis for its therapeutic development.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Endosomas / Glicoproteína de la Espiga del Coronavirus / Tratamiento Farmacológico de COVID-19 / Hidroxicolesteroles Límite: Humanos Idioma: Inglés Revista: Proc Natl Acad Sci U S A Año: 2020 Tipo del documento: Artículo

Similares

MEDLINE
LILACS
LIS
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Endosomas / Glicoproteína de la Espiga del Coronavirus / Tratamiento Farmacológico de COVID-19 / Hidroxicolesteroles Límite: Humanos Idioma: Inglés Revista: Proc Natl Acad Sci U S A Año: 2020 Tipo del documento: Artículo