The Pivotal Role of SOD1 for Prediction of COVID-19 Progression Revealed by Proteomic and Bioinformatic Strategies (preprint)

ABSTRACT

Background: Severe acute respiratory distress syndrome-associated coronavirus-2 (SARS-CoV-2) is still threatening the whole human population worldwide. Patients infected with SARS-CoV-2 present diverse symptoms regarding to the severity of the disease. Determining the proteome changes associated with diverse symptoms and in different infection stages is beneficial for clinical diagnosis and management. Methods: We performed a TMT labeling proteomic study on the plasma of healthy controls and COVID-19 patients, including those with asymptomatic infection (NS), mild syndrome (MS), and severe syndrome in the early phase (SSEP) and later phase (SSLP). Bioinformatic and ELISA were used for the data analysis and identification. Findings: Hundreds of proteins were dysregulated in the plasma of COVID-19 patients including all the clinical symptoms. Bioinformatics analysis of the dysregulated proteins revealed that oxidative stress, complement activation and glycolysis-related proteins were affected after infection with SARS-CoV-2. ELISA analysis confirmed that SOD1, PRDX2 and LDHA levels were increased along with severe symptoms and did not change after recovery compared with those in healthy controls. Both GSEA and ROC analysis indicated that SOD1 could be a pivotal player in the progression of COVID-19. Interpretation: Our results indicated that plasma proteome changes differed based on symptoms and disease stages and SOD1 could be an important protein for indicating COVID-19 progression. These results may also provide new understanding for COVID-19 diagnosis and treatment. Funding: This project is supported by Shenzhen Bay Laboratory Opening Fund, Shenzhen Key Medical Discipline Construction Fund, Guangdong Natural Science Foundation, Sanming Project of Medicine in Shenzhen.Declaration of Interests All the authors declared there are no conflicts of interest exist.Ethics Approval Statement This research was approved by the ethics committee of the Shenzhen Center for Disease Control and Prevention [Approval number 2020-025A].