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The abortive SARS-CoV-2 infection of osteoclast precursors promotes their differentiation into osteoclasts (preprint)
authorea preprints; 2024.
Preprint
en Inglés
| PREPRINT-AUTHOREA PREPRINTS | ID: ppzbmed-10.22541.au.171297675.51638761.v1
ABSTRACT
The COVID-19 pandemic has resulted in the loss of millions of lives, although a majority of those infected have managed to survive. Consequently, a set of outcomes, identified as long COVID, is now emerging. While the primary target of SARS-CoV-2 is the respiratory system, the impact of COVID-19 extends to various body parts, including the bone. This study aims to investigate the effects of acute SARS-CoV-2 infection on osteoclastogenesis, utilizing both ancestral and Omicron viral strains. Monocyte-derived macrophages (MDM), which serve as precursors to osteoclasts, were exposed to both viral variants. However, the infection proved abortive, even though ACE2 receptor expression increased post-infection, with no significant impact on cellular viability and redox balance. Both SARS-CoV-2 strains heightened osteoclast formation in a dose-dependent manner, as well as CD51/61 expression and bone resorptive ability. Notably, SARS-CoV-2 induced early pro-inflammatory M1 macrophage polarization, shifting towards an M2-like profile. Osteoclastogenesis-related genes (RANK, NFATc1, DC-STAMP, MMP9) were upregulated, and surprisingly, SARS-CoV-2 variants promoted RANKL-independent osteoclast formation. This thorough investigation illuminates the intricate interplay between SARS-CoV-2 and osteoclast precursors, suggesting potential implications for bone homeostasis and opening new avenues for therapeutic exploration in COVID-19.
Texto completo:
Disponible
Colección:
Preprints
Base de datos:
PREPRINT-AUTHOREA PREPRINTS
Asunto principal:
Enfermedades Óseas
/
Síndrome Respiratorio Agudo Grave
/
COVID-19
Idioma:
Inglés
Año:
2024
Tipo del documento:
Preprint
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