Rapid, sensitive detection of intact SARS-CoV-2 using DNA nets and a smartphone-linked fluorimeter
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
; 12387, 2023.
Article
Dans Anglais
| Scopus | ID: covidwho-20238479
ABSTRACT
We report a single-step, room-temperature, 5-10 minute SARS-CoV-2 saliva self-monitoring method that overcomes the limitations of existing approaches through the use of fluorophore-releasing Designer DNA Nanostructures (DDNs) that bind with the multivalent pattern of spike proteins on the exterior intact virions and an inexpensive smartphone-linked, pocket-size fluorimeter, called a "V-Pod” for its resemblance to an Apple AirPod™ headphone case. We characterize the V-Pod fluorimeter performance and the DDN-based assay to demonstrate a clinically relevant detection limit of 104 virus particles/mL for pseudo-typed WT SARS-CoV-2 and 105 virus particles/mL for real pathogenic variants, including Delta, Omicron, and D614g. © 2023 SPIE.
Texte intégral:
Disponible
Collection:
Bases de données des oragnisations internationales
Base de données:
Scopus
Type d'étude:
Étude diagnostique
/
Étude pronostique
Les sujets:
Variantes
langue:
Anglais
Revue:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Année:
2023
Type de document:
Article
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