Establishment and application of triple PCR method for CPV, CCoV and CRV
Journal of Southwest Minzu University Natural Science Edition
; 49(2):142-148, 2023.
Article
Dans Chinois
| CAB Abstracts | ID: covidwho-20242702
ABSTRACT
Canine parvovirus (CPV), canine coronavirus (CCoV) and canine rotavirus (CRV) are the three main causative viruses of diarrhea in dogs with similar clinical symptoms;thereby it is necessary to establish a high effective molecular detection method for differentiating the above pathogens. By optimizing the primer concentration and annealing temperature, a triple PCR method was established for simultaneous detection of CPV, CCoV and CRV, and then the specificity, sensitivity and repeatability of the method were tested. The results showed that the target fragments of CPV VP2 gene (253 bp), CCoV ORF-1b gene (379 bp) and CRV VP6 gene (852 bp) could be accurately amplified by the triple PCR method with high specificity, the detection limits of CPV, CCOV and CRV were 6.44x10-1 pg/L, 8.72x10-1 pg/L and 8.35x10-1 pg/L respectively with high sensitivity, and the method had good stability. Using this triple PCR method, 135 canine diarrhea fecal samples collected in Chengdu region from 2019 to 2020 were detected, and compared with those of single PCR method. The detection rates of CPV, CCoV and CRV were 16.30%, 20.74% and 4.44%, respectively, and the total infection rate was 51.11% (65/135) with 20.00% (13/65) co-infection rate. The detection results were consistent with three single PCR methods. In conclusion, CPV/CCoV/CRV triple PCR method successfully established in this paper can be applied as an effective molecular method to detection of related pathogens and to the epidemiological investigation.
Prion; Viral; Bacterial and Fungal Pathogens of Animals [LL821]; Diagnosis of Animal Diseases [LL886]; Genetics and Molecular Biology of Microorganisms [ZZ395]; Molecular Biology and Molecular Genetics [ZZ360]; polymerase chain reaction; genes; detection; viral diseases; dog diseases; diarrhoea; clinical aspects; open reading frames; molecular epidemiology; Canine parvovirus type 2; Canine coronavirus; dogs; China; Feline panleukopenia virus; Parvovirus; Parvovirinae; Parvoviridae; ssDNA Viruses; DNA Viruses; viruses; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; Alphacoronavirus 1; Alphacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; Canis; Canidae; Fissipeda; carnivores; mammals; vertebrates; Chordata; animals; eukaryotes; Canine rotavirus; molecular diagnosis; PCR; Canine parvovirus; People's Republic of China; viral infections; diarrhea; scouring; clinical picture; ORFs
Collection:
Bases de données des oragnisations internationales
Base de données:
CAB Abstracts
Type d'étude:
Étude diagnostique
/
Étude pronostique
langue:
Chinois
Revue:
Journal of Southwest Minzu University Natural Science Edition
Année:
2023
Type de document:
Article
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