Association of SARS-COV-2 viral RNAemia, IL- 6 gene polymorphism, serum IL-6 and peripheral blood lymphocytes and monocytes with disease severity in COVID-19 patients
Clinical Immunology
; Conference: 2023 Clinical Immunology Society Annual Meeting: Immune Deficiency and Dysregulation North American Conference. St. Louis United States. 250(Supplement) (no pagination), 2023.
Article
Dans Anglais
| EMBASE | ID: covidwho-20243635
ABSTRACT
Coronavirus disease 2019 (COVID-19) is a fatal pandemic viral disease caused by the severe acute respiratory syndrome corona virus type-2 (SARS-CoV-2). The aim of this study is to observe the associations of IL-6, SARS-COV-2 viral load (RNAemia), IL- 6 gene polymorphism and lymphocytes and monocytes in peripheral blood with disease severity in COVID-19 patients. This study was carried out from March 2021 to January 2022. RT-PCR positive 84 COVID-19 patients and 28 healthy subjects were enrolled. Blood was collected to detect SARS-COV-2 viral RNA (RNAemia) by rRT-PCR, serum IL-6 level by chemiluminescence method, SNPs of IL-6 by SSP-PCR, immunophenotyping of lymphocytes and monocyte by flow cytometry. Serum IL-6 level (pg/ml) was considerably high among critical patients (102.02 +/- 149.7) compared to severe (67.20 +/- 129.5) and moderate patients (47.04 +/- 106.5) and healthy controls (3.5 +/- 1.8). Serum SARS-CoV-2 nucleic acid positive cases detected mostly in critical patients (39.28%) and was correlated with extremely high IL-6 level and high mortality (R =.912, P < 0.001). Correlation between IL-6 and monocyte was statistically significant with disease severity (severe group, p < 0.001, and 0.867*** and critical group p < 0.001 and 0.887***). In healthy controls, moderate, severe and critically ill COVID-19 patients, IL-6 174G/C (rs 1800795) GG genotype was 82.14%, 89.20%, 67.85% and 53.57% respectively. CC and GC genotype had strong association with severity of COVID-19 when compared with GG genotype. Significant statistical difference found in genotypes between critical and moderate groups (p < 0.001, OR-10.316, CI-3.22-23.86), where CC genotype was associated with COVID-19 severity and mortality. The absolute count of T cell, B cell, NK cell, CD4+ T cells and CD8+ T cells were significantly decreased in critical group compared to healthy, moderate and severe group (P < 0.001). Exhaustion marker CD94/NKG2A was increased on NK cells and CD8+ cytotoxic T cell among critical and severe group. Absolute count of monocyte was significantly increased in critical group (P < 0.001). Serum IL-6, IL-6 174 G/C gene and SARS-CoV-2 RNAaemia can be used in clinical practice for risk assessment;T cell subsets and monocyte as biomarkers for monitoring COVID-19 severity. Monoclonal antibody targeting IL-6 receptor and NKG2A for therapeutics may prevent disease progression and decrease morbidity and mortality.Copyright © 2023 Elsevier Inc.
IL- 6 gene polymorphism; Real-time reverse transcription polymerase chain reaction assay (rRT-PCR); RNAemia; Sequence specific primer polymerase chain reaction (SSP-PCR); Single nucleotide polymorphisms (SNPs); adult; B lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; chemoluminescence; clinical assessment; clinical practice; conference abstract; controlled study; coronavirus disease 2019; critically ill patient; cytotoxic T lymphocyte; DNA polymorphism; exhaustion; female; flow cytometry; gene expression; gene frequency; genetic association; genetic marker; genetic polymorphism; genetic susceptibility; genotype; hematologic disease; human; human cell; human tissue; immunophenotyping; lymphocyte; major clinical study; male; monocyte; morbidity; mortality; natural killer cell; nonhuman; peripheral lymphocyte; prevention; protein blood level; real time reverse transcription polymerase chain reaction; risk assessment; sequence specific primer polymerase chain reaction; Severe acute respiratory syndrome coronavirus 2; single nucleotide polymorphism; T lymphocyte; viral RNA blood level; biological marker; CD94 antigen; endogenous compound; interleukin 6; interleukin 6 receptor; monoclonal antibody; natural killer cell receptor NKG2A; virus RNA
Texte intégral:
Disponible
Collection:
Bases de données des oragnisations internationales
Base de données:
EMBASE
Type d'étude:
Études expérimentales
/
Étude observationnelle
/
Étude pronostique
/
Essai contrôlé randomisé
Les sujets:
Variantes
langue:
Anglais
Revue:
Clinical Immunology
Année:
2023
Type de document:
Article
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